|
| Status |
Public on Feb 01, 2016 |
| Title |
exosomal miR BM01 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy volunteer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell type: Normal BM CD34+ cells
|
| Treatment protocol |
5 mL of supernatant was harvested. Exosomes were isolated by Exosome Precipitation Solution (ExoQuick-TC; System Biosciences, Mountain View, CA).
|
| Growth protocol |
Two hundred thausand of primary nomal BM or AML CD34+ cells were cultured on fibronectin-coated dish in the presence of cytokines including SCF, TPO, FL and Delta-like protein 4 for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted from samples using 3D-Gene RNA extraction reagent from liquid sample (Toray, Kamakura, Japan)
|
| Label |
Cy5
|
| Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Bone marrow CD34+ cell
Exosomal microRNA released from CD34+ fraction of primary BM cells.
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Dec 10, 2014 |
| Last update date |
Feb 01, 2016 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18941 |
| Series (1) |
| GSE64029 |
Exosomal microRNA released from primary normal BM and AML CD34+ cells. |
|
