|
| Status |
Public on Mar 06, 2007 |
| Title |
Cutaneous melanoma cell line EST50 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
EST50
|
| Organism |
Homo sapiens |
| Characteristics |
none
|
| Biomaterial provider |
ESTDAB Cell Bank
|
| Growth protocol |
Cells were grown according to suppliers recommendations
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using a Machery-Nagel exctraction kit
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled using the Invitrogen BioPrime array CGH labeling module
|
| |
|
| Channel 2 |
| Source name |
Promega reference male DNA
|
| Organism |
Homo sapiens |
| Characteristics |
pooled lymphoblastoid genomic DNA from ten male individuals
|
| Biomaterial provider |
Promega
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
none
|
| Label |
Cy5
|
| Label protocol |
Samples were labeled using the Invitrogen BioPrime array CGH labeling module
|
| |
|
| |
| Hybridization protocol |
Hybridized and washed as described in J÷nsson et al. (Oncogene 2007)
|
| Scan protocol |
Scanned in Agilent Microarray scanner
|
| Description |
Array CGH data for EST50 melanoma cells
|
| Data processing |
Image analysis was performed using Genepix v4.0. Resultsfile were uploaded into BASE where they were filtered and pin-based lowess normalized
|
| |
|
| Submission date |
Jan 17, 2007 |
| Last update date |
Mar 06, 2007 |
| Contact name |
Johan Staaf |
| Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
|
| Street address |
Medicon Village
|
| City |
Lund |
| ZIP/Postal code |
SE-223 81 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL4723 |
| Series (1) |
| GSE6779 |
Genomic Profiling of Malignant Melanoma using tiling resolution array CGH |
|
