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Sample GSM155243 Query DataSets for GSM155243
Status Public on May 17, 2007
Title cells grown with 2% oxygen #2
Sample type RNA
 
Source name strain PAO1 grown with 2% oxygen #2
Organism Pseudomonas aeruginosa
Characteristics Pseudomonas aeruginosa strain PAO1 grown with 2% oxygen # 2
Extracted molecule total RNA
Extraction protocol Pseudomonas aeruginosa cells were disrupted by sonication and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
Label Biotinylated GeneChip reagent
Label protocol As recommended by the manufacturer (Affymetrix)
 
Hybridization protocol As recommended by the manufacturer (Affymetrix)
Scan protocol As recommended by the manufacturer (Affymetrix)
Description cDNA synthesis, fragmentation, labeling, hybridization, and processing of Pseudomonas aeruginosa GeneChips were carried out as recommended by the manufacturer. Experiments were performed in duplicate with independently prepared samples.
Data processing The Affymetrix GeneChip Operating Software Version 1.4 was used for initial data acquisition and processing.
 
Submission date Jan 12, 2007
Last update date May 17, 2007
Contact name Carolina Alvarez
E-mail(s) alvarc@u.washington.edu
Phone 206-221-2798
Organization name University of Washington
Department Microbiology
Lab Harwood
Street address 1959 NE Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL84
Series (1)
GSE6741 Responses of Pseudomonas aeruginosa to low oxygen

Data table header descriptions
ID_REF
VALUE signal intensity
ABS_CALL detection

Data table
ID_REF VALUE ABS_CALL
AFFX-Athal_actin_at 0 A
AFFX-Athal_GAPDH_at 2.1 A
AFFX-Athal_ubq_at 0.6 A
AFFX-Bsubtilis_dapB_at 3.2 A
AFFX-Bsubtilis_lys_at 0.3 A
AFFX-Bsubtilis_pheB_at 21 P
AFFX-Bsubtilis_thrC_at 5.8 A
AFFX-Bsubtilis_trpD_at 6.7 A
AFFX-YEL002C_WPB1_at 0.5 A
AFFX-YEL018W_at 2.4 A
AFFX-YEL024W_RIP1_at 4.7 A
AFFX-YER022W_SRB4_at 0.2 A
AFFX-YER148W_SPT15_at 5.8 A
AFFX-YFL039C_ACT1_at 0.1 A
ig_1046911_1047549_at 115.2 P
ig_1047549_1046911_at 260.2 P
ig_1063544_1064555_at 43.3 P
ig_1064555_1063544_at 22.3 P
ig_1087095_1087843_at 24.2 P
ig_1087843_1087095_at 34.7 P

Total number of rows: 5900

Table truncated, full table size 110 Kbytes.




Supplementary file Size Download File type/resource
GSM155243.CEL.gz 675.0 Kb (ftp)(http) CEL
Raw data provided as supplementary file

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