|
| Status |
Public on Nov 30, 2015 |
| Title |
shRNALuc2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
sample type: Vector control
|
| Treatment protocol |
Only the transfection
|
| Growth protocol |
MCF7 cells were transiently transfected with a shRNA-NIK using lipofectamine 2000 (invitrogen) . A shRNA directed against luciferase (siRNA-Luc) was employed as a control. In addition, to overexpress NIK, MCF7 cells were transiently transfected with an expression vector (pt-REX-NIK), which contains the complete ORF of NIK. . The cells were analyzed for NIK expression 48 hours after transfection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Extraction with Trizol
|
| Label |
non
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gene expression data of MCF7shRNALUC2
|
| Data processing |
Raw CEL data were normalized using the Robust Multi-array Average (RMA) method, as implemented in the oligo package . The Limma R package was used for differential expression analysis . We identified genes with a >2-fold change and a crude p<0.05.
|
| |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
Nov 30, 2015 |
| Contact name |
Vilma Araceli Maldonado |
| E-mail(s) |
vmaldonado@inmegen.gob.mx
|
| Phone |
5553501900
|
| Organization name |
INMEGEN
|
| Department |
Investigacion
|
| Lab |
Number 3
|
| Street address |
Periferico 4809
|
| City |
México |
| State/province |
Distrito Federal |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE63382 |
Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells |
|
