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Sample GSM154620 Query DataSets for GSM154620
Status Public on Mar 08, 2007
Title piRNAs associated with Piwi from Drosophila melanogaster ovaries
Sample type SRA
 
Source name Hannon lab, Cold Spring Harbor Labs
Organism Drosophila melanogaster
Characteristics Piwi associated small RNAs isolated from ovary extracts, indicating the cloning frequency
Extracted molecule total RNA
Extraction protocol small RNA was isolated from Piwi immunoprecipitates and size separated on a 15% denaturing polyacrylamid gel.
 
Library strategy RIP-Seq
Library source transcriptomic
Library selection other
Instrument model Illumina Genome Analyzer
 
Description cDNA library was prepared according to standard small RNA cloning procedures (Pfeffer S. et al. 2005; Nat. Methods)
Data processing library was sequenced at 454 Life Sciences and small RNA were extracted bioinformatically as the sequence flanked by the two cloning linkers.
 
Submission date Jan 10, 2007
Last update date May 28, 2014
Contact name Julius Brennecke
E-mail(s) brenneck@cshl.edu
Organization name Cold Spring Harbor Labs
Lab Greg Hannon
Street address 1 Bungtown Road
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platform ID GPL9058
Series (1)
GSE6734 piRNA libraries from Drosophila melanogaster ovaries
Relations
BioSample SAMN02195377

Data table header descriptions
SEQUENCE
COUNT cloning frequency

Data table
SEQUENCE COUNT
TACGCAGAGGCCTAAGTAAATAGTC 152
TCCTCGATAGTATAGTGGTTAGTATCCC 67
TTGGACTACATATGGTTGAGGGTTGTA 47
CTTGGGAACACCGCGTGTTGTTGGCCT 47
TGCTTGGACTACATATGGTTGAGGG 44
TACGCAGAGGCCTAAGTAAATAGT 44
TCCTCGATAGTATAGTGGTTAGTATCCCC 43
TCCCACGCTCGGGCACATCTGCCTA 41
TACGCAGAGGCCTAAGTAAATAGTCCCC 39
TGCGCTGCCAAAGATAAACGAATGC 31
TCCTCGATAGTATAGTGGTTAGTATC 30
TCCAGGTGTCGAAATTGTCTTTGCC 27
TGCTTGGACTACATATGGTTGAGGGT 25
ACTACATATGGTTGAGGGTTGTA 24
TCAGGGTGACCACGCATTTCAAGGTT 23
TGCACGGTGACCGACTTTTCCGCAGC 22
TATTACGAAATGCCTGCTCTACTTT 21
TAGCTATAGACTATAAGGTGCTTGT 20
TTGGGAACACCGCGTGTTGTTGGCCT 19
TAAAAAGTCCATGACATCAGAAAGTGCC 19

Total number of rows: 13904

Table truncated, full table size 390 Kbytes.




Supplementary data files not provided
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