|
| Status |
Public on Jun 01, 2016 |
| Title |
E30VZ replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Repl.1_Ventricular Zone Embryonic day 30
|
| Organism |
Mustela putorius furo |
| Characteristics |
developmental stage: Embryonic day 30
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared with Qiagen Rneasy minikit according to manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
85ng of total RNA from each sample were amplified by Oligo-dT-T7 reverse transcription and labeled by in vitro transcription with T7 RNA polymerase in the presence of Cy3-CTP using the LowInputQuick Amp Labeling kit (Agilent) and purified using RNAeasy columns (Qiagen, Hilden, Germany)
|
| |
|
| Hybridization protocol |
After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wahs buffer 2 (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2539A scanner at 5um resolution and 100%PMT
The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction software 10.7 (Agilent).
|
| Data processing |
Raw data was corrected for background noise using the normexp method. Quantile normalization was applied to assure comparability across samples.
|
| |
|
| Submission date |
Nov 12, 2014 |
| Last update date |
Jun 02, 2016 |
| Contact name |
Victor Borrell |
| E-mail(s) |
vborrell@umh.es
|
| Organization name |
CSIC
|
| Department |
Institute of Neuroscience
|
| Lab |
221
|
| Street address |
Av Ramon y Cajal s/n
|
| City |
San Juan de Alicante |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19111 |
| Series (1) |
| GSE63203 |
A restricted period for formation of Outer Subventricular Zone defined by Cdh1 and Trnp1 levels |
|
