|
| Status |
Public on May 16, 2007 |
| Title |
Myc-IP-vs-WCE-rep2 |
| Sample type |
mixed |
| |
|
| Channel 1 |
| Source name |
Myc-H3 IP rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain: MDY510, Myc-tagged H3
|
| Treatment protocol |
Cells arrest with alpha factor. No Galactose induction
|
| Extracted molecule |
other |
| Extraction protocol |
Chromatin sheared by sonication. IPed using anti-Myc Ab (Sigma; monoclonal). Amplified with a starting amount of up to 75 ng for ChIP samples, using the DNA linear amplification
|
| Label |
Cy5
|
| Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
| |
|
| Channel 2 |
| Source name |
WCE rep2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain: MDY510, Myc-tagged H3
|
| Treatment protocol |
Cells arrest with alpha factor. No Galactose induction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin sheared by sonication.
|
| Label |
Cy3
|
| Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
| |
|
| |
| Hybridization protocol |
was hybridized according to Agilent protocol
|
| Scan protocol |
was scanned according to Agilent protocol
|
| Description |
Genome-wide levels of histone H3. Cells were arrested with alpha factor, chromatin sheared by sonication, and Myc-H3 was IPed and compared to to sonicated input (WCE)
|
| Data processing |
log2(Cy5/Cy3)
|
| |
|
| Submission date |
Jan 05, 2007 |
| Last update date |
May 16, 2007 |
| Contact name |
Tommy Kaplan |
| E-mail(s) |
tommy@cs.huji.ac.il
|
| Organization name |
Hebrew University
|
| Department |
School of Computer Science and Engineering
|
| Street address |
Givat Ram Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL4131 |
| Series (2) |
| GSE6669 |
H3 occupancy |
| GSE6680 |
Dynamics of replication-independent histone turnover in budding yeast |
|
