|
| Status |
Public on May 16, 2007 |
| Title |
PolII-IP-vs-Genomic-t60-rep1 |
| Sample type |
mixed |
| |
|
| Channel 1 |
| Source name |
PolII IP 60 minutes rep1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| Treatment protocol |
t=60 (3 hours of alpha factor, 60 minutes after galactose shift)
|
| Extracted molecule |
other |
| Extraction protocol |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| Label |
Cy5
|
| Label protocol |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| |
|
| Channel 2 |
| Source name |
Genomic 60 minutes rep1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| Treatment protocol |
t=60 (3 hours of alpha factor, 60 minutes after galactose shift)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| Label |
Cy3
|
| Label protocol |
See [Kim et al, Nature 432(7016):517-22 (2004)]
|
| |
|
| |
| Hybridization protocol |
was hybridized according to Agilent protocol
|
| Scan protocol |
was scanned according to Agilent protocol
|
| Description |
Genome-wide PolII occupancy using PolII-IP vs genomic DNA. Yeast grown on Raffinose, arrested for 3 hours using alpha factor, then shifted to Galactose. 60 minutes after Galactose induction
|
| Data processing |
log2(Cy5/Cy3)
|
| |
|
| Submission date |
Jan 05, 2007 |
| Last update date |
May 16, 2007 |
| Contact name |
Tommy Kaplan |
| E-mail(s) |
tommy@cs.huji.ac.il
|
| Organization name |
Hebrew University
|
| Department |
School of Computer Science and Engineering
|
| Street address |
Givat Ram Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL4131 |
| Series (2) |
| GSE6667 |
PolII occupancy |
| GSE6680 |
Dynamics of replication-independent histone turnover in budding yeast |
|
