|
| Status |
Public on Apr 01, 2015 |
| Title |
WL_Pish-Exp3-S3dH2O |
| Sample type |
RNA |
| |
|
| Source name |
fourth leaves of rice (pish) at 3 days after treatment with water
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar: Nipponbare
tissue: Fourth leaves
blast resistance transgene: pish
blast resistance phenotype: Susceptible
inoculant: water
|
| Treatment protocol |
Conidia suspension of rice blast fungus (1 × 106/mL) was sprayed on the fourth leaves of rice plants, which was harvested at 1, 2, 3, and 5 days after inoculation.
|
| Growth protocol |
Rice seedlings at 4.5 leaf stage were grown in a chamber under 14 h light (28C) and 10 h dark (24C).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini kit (Quiagen, #74904) was used.
|
| Label |
cy3
|
| Label protocol |
Total RNA (400 ng) was labeled with cy3 using Quick Amp Labeling Kit, One-Color (Agilent).
|
| |
|
| Hybridization protocol |
Cy3-labeled cRNA (1650 ng) was fragmented and hybridized at 65°C for 17 haccording to the manufacture's protocol.
|
| Scan protocol |
Slides were scanned on an Agilent G2505B DNA microarray scanner.
|
| Description |
Susceptible rice at 3 days after treatment with H2O (Exp3)
Gene expression in the fourth leaves of rice (pish) at 3 days after treatment with water
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.5.1.1 (Agilent) using default parameters to obtain background subtracted.
|
| |
|
| Submission date |
Oct 31, 2014 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Eiichi Minami |
| E-mail(s) |
eiminami@affrc.go.jp
|
| Phone |
1-81-29-838-7912
|
| Organization name |
National Institute of Agrobiological Sciences
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6864 |
| Series (2) |
| GSE62894 |
Gene expression in rice leaves (Pish) in response to inoculation with rice blast fungus |
| GSE62896 |
Global and local gene expression in rice leaves and roots challenged by Magnaporthe oryzae |
|
