|
| Status |
Public on Nov 28, 2023 |
| Title |
MEP_Hoxa1_r1 |
| Sample type |
RNA |
| |
|
| Source name |
MEP (group A) Hoxa1
|
| Organism |
Mus musculus |
| Characteristics |
cell population: FACS Sorted BM Lin-C-Kit+Sca-1-CD16/32-CD34- cells
pool: A
tissue: Bone Marrow
cell type: megakaryote erythorid progenitors (MEP)
overexpression: Wild type Hoxa1 (WT- Hoxa1)
background strain: C57BL/6
biological replicate: 1
|
| Growth protocol |
Cell were FACS sorted from primiary receipent mice 16 weeks post transplantation of retrovirally overexpressed immature bone marrow cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
| Scan protocol |
Performed by Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Sydney, Australia in accordance with the manufacturer’s instructions.
|
| Description |
Megakaryocyte Erythroid Progenitor Hoxa1 overexpression rep 1
|
| Data processing |
samples were RMA normalised in GenePattern using NormalizeAffymetrixST 2.2 (RMA, core probesets, controls included,Affymetrix_MoGene_1_1_st_v1.na33.2.mm9.transcript.chip).
|
| |
|
| Submission date |
Oct 30, 2014 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Alistair Morgan Chalk |
| E-mail(s) |
achalk@svi.edu.au, alistair.chalk@gmail.com
|
| Organization name |
St Vincent's Institute of Medical Research
|
| Department |
Stem Cell Regulation Unit
|
| Lab |
Walkley
|
| Street address |
9 Princes st, Fitzroy
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3065 |
| Country |
Australia |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE62853 |
Modeling myelodysplastic syndromes in mice by altered Hoxa1 spliceform expression |
|
