|
| Status |
Public on Jan 23, 2015 |
| Title |
253949410878-3 |
| Sample type |
RNA |
| |
|
| Source name |
SKOV3ipL, shNT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKOV3ipL
cell type: ovarian carcinoma cell line
shRNA: negative control (shNT)
amount in (µg): 0.2
rin number: 10
|
| Treatment protocol |
Stable knock down of CD63 was performed in SKOV3ipL ovarian carcinoma cell line using 2 shRNAs lentiviral constructs (sh49 and sh51), and as a negative control, a shNT construct. Parental cells, control shNT and the 2 shCD63 cell lines were seeded 24 hours prior to RNA isolation on a 10 cm dish.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Agilent: Low RNA Input QuickAmp.
|
| |
|
| Hybridization protocol |
Agilent: 200.0 ng at 65 degree_C during 17 hours.
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Description |
SKOV3ipL_shNT_1
|
| Data processing |
Feature extraction data processing. Quantile normalization and paired differential analysis in R / Bioconductor.
|
| |
|
| Submission date |
Oct 01, 2014 |
| Last update date |
Jan 24, 2015 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE61957 |
Tetraspanin CD63 acts as a pro-metastatic factor via β-catenin stabilization |
|
