|
| Status |
Public on Jan 15, 2015 |
| Title |
PBBA1_mRNA |
| Sample type |
RNA |
| |
|
| Source name |
CD19+ B cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Activated (IL4, anti-CD40, IgM, and IgD) peripheral blood
tumor: none
Sex: male
previously treated for nhl: NA
cell type: peripheral blood B cells
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using Qiagen RNeasy Kits according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Purified RNA was amplified using Nugen Ovation Pico SL or Ovation Pico kits and labeled using Nugen Encore Biotin kit
|
| |
|
| Hybridization protocol |
Products were hybridized to Affymetrix Human Gene 1.0 ST arrays according to the manufacturer’s protocol
|
| Scan protocol |
GS3000
|
| Description |
Gene expression analysis was performed for peripheral blood B cells using Affymetrix Human Gene 1.0 ST arrays
|
| Data processing |
Gene expression levels were determined using the Affymetrix Expression Console software (v1.2.0.20) with exon-level RMA analysis and default settings
|
| |
|
| Submission date |
Sep 30, 2014 |
| Last update date |
Jan 15, 2015 |
| Contact name |
Jacqueline Payton |
| E-mail(s) |
jpayton@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 S. Euclid Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL10739 |
| Series (2) |
| GSE61912 |
Enhancer Sequence Variants and Transcription Factor Deregulation Synergize to Construct Pathogenic Regulatory Circuits in B Cell Lymphoma (array) |
| GSE62246 |
Enhancer Sequence Variants and Transcription Factor Deregulation Synergize to Construct Pathogenic Regulatory Circuits in B Cell Lymphoma |
|
