|
| Status |
Public on Aug 14, 2015 |
| Title |
mammillary body, KO, biological rep10 |
| Sample type |
RNA |
| |
|
| Source name |
mammillary neuroepithelium of E10.5 mouse embryos
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain (mammillary neuroepithelium)
strain: C57BL/6
age: E10.5
genotype: Lhx5 KO
|
| Treatment protocol |
The mammillary neuroepithelium of E10.5 wild type and Lhx5 mutant embryos was dissected and immediately frozen at -80°C. The RNA was preserved with RNAlater ICE (Life Technologies).
|
| Growth protocol |
Mice were crossed to obtain E10.5 wild type and Lhx5 mutant embryos.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using the RNeasy Micro Kit (Qiagen) following the manufacturers instructions for animal tissue.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit
|
| |
|
| Hybridization protocol |
Hybridization was performed for 16 hr at 45°C on GeneChip Mouse Gene 1.1 ST arrays. GeneChips were washed and stained in the Affymetrix Fuidics Station 450.
|
| Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 (GSC3000) with GCOS 1.4
|
| Description |
gene expression data from E10.5 Lhx5 mutant mammillary neuroepithelium
|
| Data processing |
The data was analyzed using Expression Console 1.0 (Affymetrix) and Bioconductor package affy under R 2.7.0. Background correction, normalization and probe summarization was performed with RMA
|
| |
|
| Submission date |
Sep 22, 2014 |
| Last update date |
Aug 15, 2015 |
| Contact name |
Gonzalo Alvarez-Bolado |
| Organization name |
Institute for Anatomy and Cell Biology
|
| Street address |
Im Neuenheimer Feld 307
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11533 |
| Series (2) |
| GSE61612 |
Gene expression data from Lhx5 mutant mouse embryos |
| GSE61614 |
Identification of Lhx5 binding sites and Gene expression data from Lhx5 mutant mouse embryos |
|
