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Sample GSM1494920 Query DataSets for GSM1494920
Status Public on Nov 06, 2015
Title Duodenum_6 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: duodenal adenocarcinoma type
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen duodenal adenocarcinoma type patient 6
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.665143753
A_25_P00015343 4.72481962
Blank 4.457185718
dmr_285 12.06158814
dmr_3 14.93657175
dmr_308 4.726073499
dmr_316 4.743209125
dmr_31a 11.50752345
dmr_6 14.27714515
A_25_P00013722 4.667515638
A_25_P00013718 4.676453659
A_25_P00011668 4.660570825
A_25_P00013809 4.66576123
A_25_P00011834 4.599323246
A_25_P00013749 4.662930182
A_25_P00013752 4.793936757
A_25_P00011853 6.542405168
A_25_P00013814 4.557710305
A_25_P00014783 4.60638193
A_25_P00011886 4.609419419

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494920_miRNA_Duodenum_6.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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