|
| Status |
Public on Aug 10, 2020 |
| Title |
siRNA_Fus_2 |
| Sample type |
RNA |
| |
|
| Source name |
N2A cells transfected with siRNA_Fus_2
|
| Organism |
Mus musculus |
| Characteristics |
cell line: neuroblastoma cell line N2A
sirna: Fus_2
|
| Treatment protocol |
N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.
|
| Growth protocol |
N2A mouse neuroblastoma cells were grown in MEM with 10% fetal bovine serum at 37 ˚C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested from N2A cells in a 10-cm dish transfected with siRNA using Quickgene RNA cultured cell kit (Kurabo) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were used to generate labeled cDNA. Biotinylated DNA probes were prepared using Affymetrix WT Expression kit for Affymetrix Whole Transcript Sense Expression arrays and Affymetrix geneChip WT Terminal Labeling kit according to the manufacture's protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip mouse exon 1.0 ST Arrays (Affymetrix) and scanned accoding to the manufacture's protocol.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip 3000 7G
|
| Data processing |
The data were background corrected and normalized using the robust multiarray average (RMA) procedure implemented in the Affymetrix Expression Console software. probe group file: MoEx-1_0-st-v1.r2.pgf meta-probeset file: MoEx-1_0-st-v1.r2.dtl.mm9.full.ps
RMA expression value derived from Expression Console software; full-exon analysis
|
| |
|
| Submission date |
Aug 25, 2014 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Akio Masuda |
| E-mail(s) |
amasuda@med.nagoya-u.ac.jp
|
| Phone |
+81-52-744-2447
|
| Organization name |
Nagoya University Graduate School of Medicine
|
| Lab |
Neurogenetics
|
| Street address |
65 tsurumai-cho, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE60694 |
Exon array analysis of N2A cells after knocking down FUS |
|
