|
| Status |
Public on Aug 20, 2014 |
| Title |
#5 R+KO |
| Sample type |
RNA |
| |
|
| Source name |
b-FoxO triple KO
|
| Organism |
Mus musculus |
| Characteristics |
backgroud strain: 129 x FVBn x C57BL/6
cell type: pancreatic islets
|
| Treatment protocol |
Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
|
| Growth protocol |
All animal studies were approved by the Columbia Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeled cRNA were prepared according to the standard Nugen protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed by Columbia University microarray core facility. Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning was performed by Columbia University microarray core facility. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA by Expression Console
|
| |
|
| Submission date |
Aug 19, 2014 |
| Last update date |
Aug 20, 2014 |
| Contact name |
Domenico Accili |
| E-mail(s) |
da230@columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
1150 St. Nicholas Ave
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE60505 |
Metabolic Inflexibility Impairs Insulin Secretion And Results In MODY-like Diabetes In Triple FoxO-deficient Mice |
|
