|
| Status |
Public on Nov 23, 2006 |
| Title |
Stage 2, PT#9, Normal (HG-U133A) |
| Sample type |
RNA |
| |
|
| Source name |
Human Renal Epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
Normal, PT#9, STG2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared accordiing to the standard Affymetrix protocol from 8.4 ug of total RNA
|
| |
|
| Hybridization protocol |
Follow fragmentation, 10ug of cRNA were hybridized for 16 hrs at 45C on HG U133A and HG U133B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Description |
Normal HRE
|
| Data processing |
The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500
|
| |
|
| Submission date |
Nov 22, 2006 |
| Last update date |
Nov 22, 2006 |
| Contact name |
John A Copland |
| E-mail(s) |
Copland.John@mayo.edu
|
| Organization name |
Mayo Clinic College of Medicine
|
| Department |
Biochemistry & Molecular Biology
|
| Street address |
4500 San Pablo Road
|
| City |
Jacksonville |
| State/province |
FL |
| ZIP/Postal code |
32224 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6344 |
Gene expression in Stage 1,2 Normal and Tumor kidney cancer |
|
