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Sample GSM146376 Query DataSets for GSM146376
Status Public on Mar 31, 2007
Title Maythorpe shoot Control, biological rep 2
Sample type RNA
 
Source name Maythorpe shoot
Organism Hordeum vulgare
Characteristics Genotype: Maythorpe

Treatment protocol control shoot
Growth protocol hydroponics
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description Gene expression data from barley shoot
Data processing The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 20, 2006
Last update date Mar 31, 2007
Contact name Harkamal Walia
Organization name University of Nebraska
Street address Agronomy and Horticulture
City Lincoln
State/province NE
ZIP/Postal code 68583
Country USA
 
Platform ID GPL1340
Series (1)
GSE6325 Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise

Data table header descriptions
ID_REF
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 181.1 P 0.002023
AFFX-BioB-M_at 105.4 P 0.000127
AFFX-BioB-3_at 118.5 P 0.00034
AFFX-BioC-5_at 356.5 P 0.00007
AFFX-BioC-3_at 507.6 P 0.000044
AFFX-BioDn-5_at 668.8 P 0.000052
AFFX-BioDn-3_at 2089.7 P 0.00011
AFFX-CreX-5_at 3583.2 P 0.000044
AFFX-CreX-3_at 6355.8 P 0.000044
AFFX-DapX-5_at 270.8 P 0.000052
AFFX-DapX-M_at 802.6 P 0.00039
AFFX-DapX-3_at 1459.3 P 0.000044
AFFX-LysX-5_at 39 P 0.00007
AFFX-LysX-M_at 127.6 P 0.006871
AFFX-LysX-3_at 247.9 P 0.000127
AFFX-PheX-5_at 103.8 P 0.001794
AFFX-PheX-M_at 88.5 P 0.004484
AFFX-PheX-3_at 184 P 0.00007
AFFX-ThrX-5_at 107.6 P 0.001796
AFFX-ThrX-M_at 249.6 P 0.00011

Total number of rows: 22840

Table truncated, full table size 702 Kbytes.




Supplementary file Size Download File type/resource
GSM146376.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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