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Sample GSM146375 Query DataSets for GSM146375
Status Public on Mar 31, 2007
Title Maythorpe shoot Control, biological rep1
Sample type RNA
 
Source name Maythorpe shoot
Organism Hordeum vulgare
Characteristics Genotype: Maythorpe

Treatment protocol control shoot
Growth protocol hydroponics
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description Gene expression data from barley shoot
Data processing The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Nov 20, 2006
Last update date Mar 31, 2007
Contact name Harkamal Walia
Organization name University of Nebraska
Street address Agronomy and Horticulture
City Lincoln
State/province NE
ZIP/Postal code 68583
Country USA
 
Platform ID GPL1340
Series (1)
GSE6325 Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise

Data table header descriptions
ID_REF
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 171.5 P 0.003595
AFFX-BioB-M_at 103.2 P 0.000581
AFFX-BioB-3_at 65.3 P 0.002023
AFFX-BioC-5_at 288.3 P 0.000095
AFFX-BioC-3_at 413.7 P 0.000044
AFFX-BioDn-5_at 520.3 P 0.00007
AFFX-BioDn-3_at 1532.5 P 0.000169
AFFX-CreX-5_at 2762.1 P 0.000044
AFFX-CreX-3_at 4679.4 P 0.000044
AFFX-DapX-5_at 140.9 P 0.000095
AFFX-DapX-M_at 431 P 0.000509
AFFX-DapX-3_at 881.5 P 0.000044
AFFX-LysX-5_at 33.8 P 0.000581
AFFX-LysX-M_at 86.1 P 0.030976
AFFX-LysX-3_at 125.2 P 0.000147
AFFX-PheX-5_at 68.8 P 0.00034
AFFX-PheX-M_at 55.8 P 0.036569
AFFX-PheX-3_at 99.2 P 0.000127
AFFX-ThrX-5_at 90 P 0.003595
AFFX-ThrX-M_at 131.5 P 0.000297

Total number of rows: 22840

Table truncated, full table size 702 Kbytes.




Supplementary file Size Download File type/resource
GSM146375.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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