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GEO accession:
Sample GSM146375
Query DataSets for GSM146375
Status
Public on Mar 31, 2007
Title
Maythorpe shoot Control, biological rep1
Sample type
RNA
Source name
Maythorpe shoot
Organism
Hordeum vulgare
Characteristics
Genotype: Maythorpe
Treatment protocol
control shoot
Growth protocol
hydroponics
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description
Gene expression data from barley shoot
Data processing
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
Submission date
Nov 20, 2006
Last update date
Mar 31, 2007
Contact name
Harkamal Walia
Organization name
University of Nebraska
Street address
Agronomy and Horticulture
City
Lincoln
State/province
NE
ZIP/Postal code
68583
Country
USA
Platform ID
GPL1340
Series (1)
GSE6325
Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise
Data table header descriptions
ID_REF
VALUE
GCOS-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
171.5
P
0.003595
AFFX-BioB-M_at
103.2
P
0.000581
AFFX-BioB-3_at
65.3
P
0.002023
AFFX-BioC-5_at
288.3
P
0.000095
AFFX-BioC-3_at
413.7
P
0.000044
AFFX-BioDn-5_at
520.3
P
0.00007
AFFX-BioDn-3_at
1532.5
P
0.000169
AFFX-CreX-5_at
2762.1
P
0.000044
AFFX-CreX-3_at
4679.4
P
0.000044
AFFX-DapX-5_at
140.9
P
0.000095
AFFX-DapX-M_at
431
P
0.000509
AFFX-DapX-3_at
881.5
P
0.000044
AFFX-LysX-5_at
33.8
P
0.000581
AFFX-LysX-M_at
86.1
P
0.030976
AFFX-LysX-3_at
125.2
P
0.000147
AFFX-PheX-5_at
68.8
P
0.00034
AFFX-PheX-M_at
55.8
P
0.036569
AFFX-PheX-3_at
99.2
P
0.000127
AFFX-ThrX-5_at
90
P
0.003595
AFFX-ThrX-M_at
131.5
P
0.000297
Total number of rows:
22840
Table truncated, full table size
702 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM146375.CEL.gz
3.4 Mb
(ftp)
(http)
CEL
Raw data provided as supplementary file
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