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GEO accession:
Sample GSM146371
Query DataSets for GSM146371
Status
Public on Mar 31, 2007
Title
Golden Promise shoot Control, biological rep 3
Sample type
RNA
Source name
Golden Promise shoot
Organism
Hordeum vulgare
Characteristics
Genotype: Golden Promise
Treatment protocol
control shoot
Growth protocol
hydroponics
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner
Description
Gene expression data from barley shoot
Data processing
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
Submission date
Nov 20, 2006
Last update date
Mar 31, 2007
Contact name
Harkamal Walia
Organization name
University of Nebraska
Street address
Agronomy and Horticulture
City
Lincoln
State/province
NE
ZIP/Postal code
68583
Country
USA
Platform ID
GPL1340
Series (1)
GSE6325
Array-based genotyping and expression analysis of barley cv. Maythorpe and Golden Promise
Data table header descriptions
ID_REF
VALUE
GCOS-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
172.7
P
0.000754
AFFX-BioB-M_at
98.3
P
0.000258
AFFX-BioB-3_at
102.3
P
0.000446
AFFX-BioC-5_at
360.7
P
0.000044
AFFX-BioC-3_at
570.4
P
0.000052
AFFX-BioDn-5_at
654.2
P
0.000052
AFFX-BioDn-3_at
1855.2
P
0.000095
AFFX-CreX-5_at
3332.4
P
0.000044
AFFX-CreX-3_at
5999.4
P
0.000044
AFFX-DapX-5_at
211.8
P
0.000081
AFFX-DapX-M_at
672.9
P
0.000225
AFFX-DapX-3_at
1488.9
P
0.000052
AFFX-LysX-5_at
53.4
P
0.000147
AFFX-LysX-M_at
105.7
P
0.013811
AFFX-LysX-3_at
270.6
P
0.00006
AFFX-PheX-5_at
90.4
P
0.000297
AFFX-PheX-M_at
58.1
P
0.003595
AFFX-PheX-3_at
170.1
P
0.000127
AFFX-ThrX-5_at
83.4
P
0.001102
AFFX-ThrX-M_at
222.7
P
0.000081
Total number of rows:
22840
Table truncated, full table size
703 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM146371.CEL.gz
3.4 Mb
(ftp)
(http)
CEL
Raw data provided as supplementary file
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