NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1448187 Query DataSets for GSM1448187
Status Public on Jun 01, 2015
Title KM12L4
Sample type RNA
 
Source name KM12L4 CRC cell line, standard culture conditions
Organism Homo sapiens
Characteristics cell line: KM12L4
Treatment protocol No treatment
Growth protocol Standard culture conditions for each cell line
Extracted molecule total RNA
Extraction protocol total RNA was extracted using miRNeasy Mini Kit (Qiagen), according to the manufacturer’s protocol, without the separation step between mRNAs and microRNAs
Label Biotin
Label protocol Synthesis of cDNA and biotinylated cRNA was performed using the IlluminaTotalPrep RNA Amplification Kit (Ambion), according to the manufacturer’s protocol using 500ng of total RNA
 
Hybridization protocol Hybridization of cRNAs (750 ng) was carried out using Illumina Human 48k gene chips (Human HT-12 V4 BeadChip)
Scan protocol Hybridized Beadchips were scanned with the Illumina BeadArray Reader
Description CRC cell line
Data processing Non-normalized data were generated by the Illumina GenomeStudio software version V2011.1 . Data were Loess normalized with the Lumi R package
Signal values and detection p-values as provided by the Illumina Genome Studio software. Normalized signal values as provided by the Lumi R package.
 
Submission date Jul 29, 2014
Last update date Jun 01, 2015
Contact name Enzo Medico
E-mail enzo.medico@ircc.it
Phone +39-011-9933234
Organization name Candiolo Cancer Institute, University of Torino
Department Oncology
Lab Laboratory of Oncogenomics
Street address Strada Prov. 142, km 3,95
City Candiolo
State/province TO
ZIP/Postal code 10060
Country Italy
 
Platform ID GPL10558
Series (1)
GSE59857 Global gene expression profiling of human colorectal cancer cell lines

Data table header descriptions
ID_REF
VALUE Loess normalized signal
9371160028_G.Detection Pval

Data table
ID_REF VALUE 9371160028_G.Detection Pval
ILMN_3166687 165.4964857 0.4987
ILMN_3165565 169.8612352 0.48052
ILMN_3164808 136.4554915 0.9026
ILMN_3165363 142.3206253 0.84416
ILMN_3166504 171.3480553 0.45974
ILMN_3164750 132.0251822 0.94805
ILMN_3166430 171.3265288 0.42727
ILMN_3165745 152.7267074 0.68571
ILMN_3164915 156.9473289 0.62987
ILMN_3165415 134.1854795 0.9039
ILMN_3165201 145.7898686 0.8039
ILMN_3166630 172.3202827 0.37273
ILMN_3165619 123.9072161 0.97013
ILMN_3165933 166.6137678 0.53247
ILMN_3164979 179.6698935 0.27792
ILMN_3166789 176.6975373 0.37143
ILMN_3165033 133.4695168 0.94286
ILMN_3165699 143.2141527 0.83117
ILMN_3165218 117.9474701 0.98961
ILMN_3166404 168.6641104 0.4974

Total number of rows: 47323

Table truncated, full table size 1453 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data is available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap