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Sample GSM1446743 Query DataSets for GSM1446743
Status Public on Jul 29, 2014
Title Cell line MO7E
Sample type RNA
 
Source name Cell line MO7E
Organism Homo sapiens
Characteristics cell line: MO7E
Growth protocol NOMO1, KG1, PLB985, ML2, HL-60, EOL1, FKH1, GDM1, HNT34, THP1, SKM1, PL21, ME-1, KMOE-2, GF-D8, HEL, SET2, KG1?, KASUMI-1, MO7e and NB4 were grown in RPMI 1640 medium supplemented with 10% or 20% FBS; F-36P was cultured in RPMI 1640 medium supplemented with 10% FBS and 5ng/ml human IL-3; OCI-M1, OCI-M2, SHI-1, and SH-2 were cultured in IMDM medium supplemented with 20% FBS. OCI-AML2, OCI-AML5, OCI-AML3 were cultured in alpha-MEM medium supplemented with 10% or 20% FBS and GM-CSF or SCF. All cell lines were placed in a 37C humidified incubator containing 5% CO2. 10x10^6 cells were washed with 1xPBS and the cell pellets were stored at -80C before sending out for Microarray analysis.
Extracted molecule total RNA
Extraction protocol Total RNA is isolated using the Qiagen miRNeasy Mini Kit (Qiagen, #217004).
Label Biotin
Label protocol RNA samples are converted into labeled target antisense RNA (cRNA) using the Single-Round RNA Amplification and Biotin Labelling System (Enzo).
 
Hybridization protocol cRNA is purified using magnetic beads and quantitated using spectrophotometry. Next, 11ug of purified cRNA is fragmented using a 5X Fragmentation buffer, then a hybridization cocktail is prepared and added to the fragmentation product using the Hybridization, Wash and Stain kit (Affymetrix), applied to Human U133_Plus2.0 arrays, and incubated at 45°C for 16 hours.
Scan protocol Following hybridization, arrays are washed and stained using standard Affymetrix procedures before scanning on the Affymetrix GeneChip Scanner
Description Gene expression data from AML cell line
Data processing Data extraction was performed using Expression Console
 
Submission date Jul 28, 2014
Last update date Jul 29, 2014
Contact name Shih-Min A Huang
E-mail(s) alexhuang001@gmail.com
Organization name Sanofi Oncology
Department Discovery and Early Development
Street address 640 Memorial Drive
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL570
Series (1)
GSE59808 Expression data from AML cell lines

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
Detect_149882
PValue_149882

Data table
ID_REF VALUE Detect_149882 PValue_149882
AFFX-BioB-5_at 1273.1 P 0.0005
AFFX-BioB-M_at 1635.6 P 0.0000
AFFX-BioB-3_at 1063.5 P 0.0000
AFFX-BioC-5_at 2050.2 P 0.0001
AFFX-BioC-3_at 2162.2 P 0.0000
AFFX-BioDn-5_at 3644.3 P 0.0000
AFFX-BioDn-3_at 13337.3 P 0.0001
AFFX-CreX-5_at 29390.7 P 0.0001
AFFX-CreX-3_at 32690.3 P 0.0000
AFFX-DapX-5_at 14.7 A 0.3969
AFFX-DapX-M_at 18.5 A 0.6031
AFFX-DapX-3_at 8.4 A 0.6729
AFFX-LysX-5_at 15.0 A 0.3826
AFFX-LysX-M_at 4.2 A 0.7249
AFFX-LysX-3_at 1.8 A 0.5886
AFFX-PheX-5_at 3.1 A 0.5446
AFFX-PheX-M_at 17.6 A 0.4114
AFFX-PheX-3_at 7.9 A 0.9164
AFFX-ThrX-5_at 23.7 A 0.5446
AFFX-ThrX-M_at 7.1 A 0.6863

Total number of rows: 54675

Table truncated, full table size 1350 Kbytes.




Supplementary file Size Download File type/resource
GSM1446743_EA11075_149882_HG-U133_PLUS_2_MO7E.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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