|
| Status |
Public on Oct 06, 2014 |
| Title |
control HUES26 H3K4me3 |
| Sample type |
genomic |
| |
|
| Source name |
control HUES26 H3K4me3
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: none
cell line: HuES26
chip antibody: K4me
|
| Treatment protocol |
cells were treated with 10 ng/ml BMP2 for two days
|
| Growth protocol |
HUES cells were cultured as previously described (Stefanovic et al J cell biol 2009 186(5):665-73.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
cells were crosslinked and chromatin extracted as previously described (Zeineddine et al Dev Cell. 2006 ,11(4):535-46.)
|
| Label |
biotin
|
| Label protocol |
Samples were prepared and hybridized to Gne ChiP Human promoter Tiling 1.0R Array according to Affymetrix specifications.
|
| |
|
| Hybridization protocol |
Samples were prepared and hybridized to Gne ChiP Human promoter Tiling 1.0R Array according to Affymetrix specifications.
|
| Scan protocol |
The tiling arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G according to manufacturer's instruction.
|
| Description |
results for K4meHuES26
|
| Data processing |
The signal intensity data was analyzed with the Affymetrix Tiling Analysis Software (v. 1.1.02) the identified binding sites were mapped to the gene promoter sites using Genomatrix
the identified binding sites were mapped to the gene promoter sites using Genomatrix
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Oct 06, 2014 |
| Contact name |
michel PUCEAT |
| E-mail(s) |
michel.puceat@inserm.fr
|
| Phone |
33 4 91 32 49 47
|
| Organization name |
INSERM
|
| Department |
University aix marseille
|
| Lab |
physiopathology of cardiac development
|
| Street address |
27 bd Jean moulin
|
| City |
Marseille |
| ZIP/Postal code |
13885 |
| Country |
France |
| |
|
| Platform ID |
GPL5082 |
| Series (1) |
| GSE59328 |
Genome wide analysis of bivalent domains in two HUES cell lines |
|
