|
| Status |
Public on Feb 24, 2015 |
| Title |
2BS2RL-RNA-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
The third leaf stage, RNA
|
| Organism |
Triticum aestivum |
| Characteristics |
genotype/variation: Hexaploid wheat-rye translocation
|
| Growth protocol |
Seeds were planted in Sunshine Mix #1 (Sun Gro, Canada) and maintained in a plant growth chamber with an average day/night temperature of 23/20°C, 14 h of light (12,000 LUX), and a relative humidity of 60% for 10 days. When plants reached the third leaf stage, the whole seedling including leaf and roots were harvested for RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from seedlings using TRIzol reagent (Invitrogen, USA), according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by GreenGene Biotech Inc (http://www.ggbio.com), Republic of Korea following their standard operating protocol.
|
| Scan protocol |
Scanning was performed by GreenGene Biotech Inc (http://www.ggbio.com), Republic of Korea following their standard operating protocol.
|
| Description |
This sample is of 2BS.2RL wheat-rye translocation. It is the first of two biological replicates used in this experiment.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis).
|
| |
|
| Submission date |
Jun 19, 2014 |
| Last update date |
Feb 24, 2015 |
| Contact name |
Tong Geon Lee |
| E-mail(s) |
agrospel@illinois.edu
|
| Organization name |
University of Illinois
|
| Street address |
1101 W Peabody
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL18847 |
| Series (1) |
| GSE58678 |
Expression analysis of individual homoeologous wheat genome- and rye genome-specific transcripts in a 2BS.2RL wheat-rye translocation |
|
