strain: C57BL/6J tissue: sclera phenotype: myopic age: 34 days
Treatment protocol
C57BL/6J mice were purchased from the Jackson Laboratory. Form-deprivation was induced in fifteen postnatal day 24 (P24) mice by applying a unilateral frosted hemispherical plastic diffuser over the right eyes for ten days. The contralateral untreated left eyes were used as control. At the end of form-deprivation induction (P34) both myopic and control eyes were isolated and retina and sclera were dissected out in RNAlatter.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from the whole eye, retina and sclera using mirVanaTM miRNA kit (Applied Biosystems, Austin, Texas) according to the manufacturer’s instructions. Total RNA concentration was measured using NanoDrop spectrophotometry. The quality of the total RNA was assessed using the Agilent RNA 600 Nano kit (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions.
Label
Cy3
Label protocol
Agilent miRNA Microarray System with miRNA Complete Labeling and Hyb Kit was used for the labeling protocol.
Hybridization protocol
Agilent miRNA Microarray System with miRNA Complete Labeling and Hyb Kit for the hybridization protocol. Ozone covers were used.
Scan protocol
Agilent B Scanner, Scan area (61 x 21.6mm), Scan resolution 5um, extended Dynamic range selected, Dye channel green, Green PMT XDR Hi 100%, XDR Lo 5%
Description
miRNA expression after 10 days of FDM induction MmiRNA-S-C-001
Data processing
Feature Extraction version 11, protocol miRNA_1100_Jul11
