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Sample GSM139923 Query DataSets for GSM139923
Status Public on Oct 14, 2006
Title Anther telophaseI-telophaseII rep2
Sample type RNA
 
Source name whole wheat anther, telophaseI-telophaseII
Organism Triticum aestivum
Characteristics Cultivar: Chinese Spring
Tissue: Anther
Stage: TelophaseI-TelophaseII
Biomaterial provider Able Laboratory - University of Adelaide
Treatment protocol Nil
Growth protocol Wild-type wheat was grown in a temperature-controlled glass house at 23 degrees Celsius (day) and 15 degrees Celsius (night) with a 14 hour photoperiod.
Extracted molecule total RNA
Extraction protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Label Biotin
Label protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
 
Hybridization protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Scan protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Description Our understanding of the mechanisms that govern the cellular process of
meiosis is limited in higher plants with polyploid genomes. Bread wheat is an allohexaploid that behaves as a diploid during meiosis. Chromosome pairing is restricted to homologous chromosomes despite the presence of homoeologues in the nucleus. The importance of wheat as a crop and the extensive use of wild wheat relatives in breeding programs has prompted many years of cytogenetic and genetic research to develop an understanding of the control of chromosome pairing and recombination. The rapid advance of biochemical and molecular information on meiosis in model organisms such as yeast provides new opportunities to investigate the molecular basis of chromosome pairing control in wheat. However, building the link between the model and wheat requires points of data contact. We report here a large-scale transcriptomics study using the Affymetrix wheat GeneChipĀ® aimed at providing this link between wheat and model systems and at identifying early meiotic genes. Analysis of the microarray data identified 1,350
transcripts temporally-regulated during the early stages of meiosis. Expression profiles with annotated transcript functions including chromatin condensation, synaptonemal complex formation,recombination and fertility were identified. From the 1,350 transcripts, 30 displayed at least an eight-fold expression change between and including pre-meiosis and telophase II, with more than 50% of these having no similarities to
known sequences in NCBI and TIGR databases. This resource is now available to support research into the molecular basis of pairing and recombination control in the complex polyploid, wheat.
Data processing RMA normalized (not log scaled)
 
Submission date Oct 13, 2006
Last update date Oct 13, 2006
Contact name Jason Alan Able
E-mail(s) jason.able@adelaide.edu.au
Organization name The University of Adelaide
Department School of Agriculture, Food & Wine
Street address Waite Main Building
City Adelaide
State/province SA
ZIP/Postal code 5064
Country Australia
 
Platform ID GPL3802
Series (1)
GSE6027 Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

Data table header descriptions
ID_REF
VALUE TelophaseI-TelophaseII2

Data table
ID_REF VALUE
AFFX-BioB-3_at 163.4755159
AFFX-BioB-5_at 190.1639918
AFFX-BioB-M_at 175.711843
AFFX-BioC-3_at 465.8285295
AFFX-BioC-5_at 384.2629889
AFFX-BioDn-3_at 2026.303566
AFFX-BioDn-5_at 872.5946066
AFFX-CreX-3_at 8599.218144
AFFX-CreX-5_at 6067.55951
AFFX-DapX-3_at 982.0604816
AFFX-DapX-5_at 31.28342017
AFFX-DapX-M_at 239.7573878
AFFX-LysX-3_at 307.2384386
AFFX-LysX-5_at 19.67655567
AFFX-LysX-M_at 49.1745921
AFFX-PheX-3_at 195.8079118
AFFX-PheX-5_at 25.34164258
AFFX-PheX-M_at 59.0365611
AFFX-r2-Bs-dap-3_at 1160.408576
AFFX-r2-Bs-dap-5_at 24.66144148

Total number of rows: 61290

Table truncated, full table size 1861 Kbytes.




Supplementary file Size Download File type/resource
GSM139923.CEL.gz 8.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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