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Sample GSM139920 Query DataSets for GSM139920
Status Public on Oct 14, 2006
Title Anther diplotene-anaphaseI rep2
Sample type RNA
 
Source name whole wheat anther, diplotene-anaphaseI
Organism Triticum aestivum
Characteristics Cultivar: Chinese Spring
Tissue: Anther
Stage: Diplotene-AnaphaseI
Biomaterial provider Able Laboratory - University of Adelaide
Treatment protocol Nil
Growth protocol Wild-type wheat was grown in a temperature-controlled glass house at 23 degrees Celsius (day) and 15 degrees Celsius (night) with a 14 hour photoperiod.
Extracted molecule total RNA
Extraction protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Label Biotin
Label protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
 
Hybridization protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Scan protocol Crismani W, Baumann U, Sutton T, Shirley N, Webster T, Spangenberg G, Langridge P, Able JA (2006) 'Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat', BMC Genomics (in press)
Description Our understanding of the mechanisms that govern the cellular process of
meiosis is limited in higher plants with polyploid genomes. Bread wheat is an allohexaploid that behaves as a diploid during meiosis. Chromosome pairing is restricted to homologous chromosomes despite the presence of homoeologues in the nucleus. The importance of wheat as a crop and the extensive use of wild wheat relatives in breeding programs has prompted many years of cytogenetic and genetic research to develop an understanding of the control of chromosome pairing and recombination. The rapid advance of biochemical and molecular information on meiosis in model organisms such as yeast provides new opportunities to investigate the molecular basis of chromosome pairing control in wheat. However, building the link between the model and wheat requires points of data contact. We report here a large-scale transcriptomics study using the Affymetrix wheat GeneChip® aimed at providing this link between wheat and model systems and at identifying early meiotic genes. Analysis of the microarray data identified 1,350
transcripts temporally-regulated during the early stages of meiosis. Expression profiles with annotated transcript functions including chromatin condensation, synaptonemal complex formation,recombination and fertility were identified. From the 1,350 transcripts, 30 displayed at least an eight-fold expression change between and including pre-meiosis and telophase II, with more than 50% of these having no similarities to
known sequences in NCBI and TIGR databases. This resource is now available to support research into the molecular basis of pairing and recombination control in the complex polyploid, wheat.
Data processing RMA normalized (not log scaled)
 
Submission date Oct 13, 2006
Last update date Oct 13, 2006
Contact name Jason Alan Able
E-mail(s) jason.able@adelaide.edu.au
Organization name The University of Adelaide
Department School of Agriculture, Food & Wine
Street address Waite Main Building
City Adelaide
State/province SA
ZIP/Postal code 5064
Country Australia
 
Platform ID GPL3802
Series (1)
GSE6027 Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

Data table header descriptions
ID_REF
VALUE Diplotene-AnaphaseI2

Data table
ID_REF VALUE
AFFX-BioB-3_at 238.5314948
AFFX-BioB-5_at 239.5178923
AFFX-BioB-M_at 242.526197
AFFX-BioC-3_at 665.0495939
AFFX-BioC-5_at 486.5586016
AFFX-BioDn-3_at 2309.2269
AFFX-BioDn-5_at 1130.111503
AFFX-CreX-3_at 9211.731641
AFFX-CreX-5_at 6699.122875
AFFX-DapX-3_at 2054.948143
AFFX-DapX-5_at 93.62161488
AFFX-DapX-M_at 671.485336
AFFX-LysX-3_at 612.7784666
AFFX-LysX-5_at 31.71682869
AFFX-LysX-M_at 167.1924499
AFFX-PheX-3_at 469.8767128
AFFX-PheX-5_at 48.33000669
AFFX-PheX-M_at 166.7591889
AFFX-r2-Bs-dap-3_at 2494.014641
AFFX-r2-Bs-dap-5_at 74.47431439

Total number of rows: 61290

Table truncated, full table size 1861 Kbytes.




Supplementary file Size Download File type/resource
GSM139920.CEL.gz 8.3 Mb (ftp)(http) CEL

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