|
| Status |
Public on Nov 24, 2014 |
| Title |
AHR_knockdown_DMSO_treated_6hpd_replicate2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Embryonic heart_AHR_Knockdown__6hpd
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Embryonic_heart
knockdown: AHR_knockdown
time extracted: 6hpd
treatment: DMSO
|
| Treatment protocol |
AB zebrafish (cmlc2::GFP) were maintained according to Nusslein-Volhard (2002), and embryos were obtained for morpholino injections at 24 hpf and exposed to control (DMSO), BaP, FL and BaP+FL at 36 hpf. Hearts were extracted at 2, 6, 12, and 18 hours post dosing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
| |
|
| Channel 2 |
| Source name |
Pooled RNA from embryonic fibroblast cell line, Zf4 cells derived from one-day old zebrafish embryos
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Fiberblast_Zf4 cells
age: 24h embryos
|
| Treatment protocol |
AB zebrafish (cmlc2::GFP) were maintained according to Nusslein-Volhard (2002), and embryos were obtained for morpholino injections at 24 hpf and exposed to control (DMSO), BaP, FL and BaP+FL at 36 hpf. Hearts were extracted at 2, 6, 12, and 18 hours post dosing.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
| |
|
| |
| Hybridization protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
| Scan protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
| Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 23, 2014 |
| Last update date |
Nov 24, 2014 |
| Contact name |
Nishad Jayasundara |
| E-mail(s) |
jnishad@gmail.com
|
| Phone |
5107783140
|
| Organization name |
Duke University
|
| Department |
Nicholas School of the Environment
|
| Lab |
Di Giulio Laboratory
|
| Street address |
A346, Research Drive, LSRC
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL18725 |
| Series (1) |
| GSE57946 |
Role of AHR in cardiac developmental toxicity of PAHs |
|
