|
Status |
Public on Nov 24, 2014 |
Title |
AHR_knockdown_BaP+FL_treated_2hpd_replicate3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Embryonic heart_AHR_Knockdown__2hpd
|
Organism |
Danio rerio |
Characteristics |
tissue: Embryonic_heart knockdown: AHR_knockdown time extracted: 2hpd treatment: BaP+FL
|
Treatment protocol |
AB zebrafish (cmlc2::GFP) were maintained according to Nusslein-Volhard (2002), and embryos were obtained for morpholino injections at 24 hpf and exposed to control (DMSO), BaP, FL and BaP+FL at 36 hpf. Hearts were extracted at 2, 6, 12, and 18 hours post dosing.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
|
|
Channel 2 |
Source name |
Pooled RNA from embryonic fibroblast cell line, Zf4 cells derived from one-day old zebrafish embryos
|
Organism |
Danio rerio |
Characteristics |
tissue: Fiberblast_Zf4 cells age: 24h embryos
|
Treatment protocol |
AB zebrafish (cmlc2::GFP) were maintained according to Nusslein-Volhard (2002), and embryos were obtained for morpholino injections at 24 hpf and exposed to control (DMSO), BaP, FL and BaP+FL at 36 hpf. Hearts were extracted at 2, 6, 12, and 18 hours post dosing.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
|
|
|
Hybridization protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
Scan protocol |
Standard Aglient protocol by Cogenics – Beckman Coulter Genomics
|
Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
May 23, 2014 |
Last update date |
Nov 24, 2014 |
Contact name |
Nishad Jayasundara |
E-mail(s) |
jnishad@gmail.com
|
Phone |
5107783140
|
Organization name |
Duke University
|
Department |
Nicholas School of the Environment
|
Lab |
Di Giulio Laboratory
|
Street address |
A346, Research Drive, LSRC
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
|
|
Platform ID |
GPL18725 |
Series (1) |
GSE57946 |
Role of AHR in cardiac developmental toxicity of PAHs |
|