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Sample GSM1385015 Query DataSets for GSM1385015
Status Public on Jul 29, 2014
Title Human SZ95 sebocytes_control vs 10 μM CBD-treated (24hrs)_rep2-1 (technical repeat of rep2)
Sample type RNA
 
Channel 1
Source name SZ95 sebocytes_vehicle
Organism Homo sapiens
Characteristics cell line: SZ95
cell type: immortalized human sebaceous gland cells
treated with: ethanol for 24hr (vehicle control)
Treatment protocol Cells were seeded to Petri-dishes. At cca. 60% confluence, cells were treated by CBD (10 μM) or by vehicle (ethanol) for 24 hrs.
Growth protocol Human immortalized SZ95 sebocytes, originated from human facial sebaceous glands (Zouboulis et al, J Invest Dermatol, 1999), were cultured in Sebomed® Basal Medium (Biochrom) supplemented with 10% fetal bovine serum (LifeTechnologies), 1 mM CaCl2, 5 ng/ml human epidermal growth factor (Sigma-Aldrich), 50 IU/ml penicillin and 50 μg/ml streptomycin (both from Teva).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using Trizol following manufacturer's instructions
Label Cy3
Label protocol RNA samples (200 ng of each) were amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol, with Spike-In controls
 
Channel 2
Source name SZ95 sebocytes_CBD
Organism Homo sapiens
Characteristics cell line: SZ95
cell type: immortalized human sebaceous gland cells
treated with: 10 μM (-)-cannabidiol (CBD) for 24hrs
Treatment protocol Cells were seeded to Petri-dishes. At cca. 60% confluence, cells were treated by CBD (10 μM) or by vehicle (ethanol) for 24 hrs.
Growth protocol Human immortalized SZ95 sebocytes, originated from human facial sebaceous glands (Zouboulis et al, J Invest Dermatol, 1999), were cultured in Sebomed® Basal Medium (Biochrom) supplemented with 10% fetal bovine serum (LifeTechnologies), 1 mM CaCl2, 5 ng/ml human epidermal growth factor (Sigma-Aldrich), 50 IU/ml penicillin and 50 μg/ml streptomycin (both from Teva).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using Trizol following manufacturer's instructions
Label Cy5
Label protocol RNA samples (200 ng of each) were amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol, with Spike-In controls
 
 
Hybridization protocol The hybridization procedure was performed according to the Two-color Microarray-Based Gene Expression Analysis (Qick Amp Labeling) protocol using the Agilent Gene Expression Hybridization Kit. Briefly, 825 ng of the corresponding Cy3- and Cy5-labeled cRNA were combined and hybridized overnight (17 hours, 65 °C) to Agilent Whole Human Genome 4x44K Oligo Microarrays using Agilent’s recommended hybridization chamber and oven.
Scan protocol Fluorescence signals of Microarrays were detected by Agilent’s Microarray Scanner.
Description Second microarray of Sample set 2
Data processing To read out and process the microarray image files the Agilent Feature Extraction Software was used. R/BioConductor software (R version: 2.15.1, limma package) was used for background subtraction and LOWESS normalization. Extracted channels were quantile normalized. Weights of features were set according to Agilent's recommendations.
 
Submission date May 12, 2014
Last update date Jul 29, 2014
Contact name Attila Oláh
E-mail(s) olah.lcmp@gmail.com
Organization name University of Debrecen
Department Department of Physiology
Street address Nagyerdei krt. 98.
City Debrecen
ZIP/Postal code H-4032
Country Hungary
 
Platform ID GPL4133
Series (1)
GSE57571 Effects of (-)-cannabidiol on human sebocytes

Data table header descriptions
ID_REF
VALUE Normalized log2 ratio (Cy3/Cy5) representing reference/test

Data table
ID_REF VALUE
12 -1.14833464
13 0.74284578
14 0.50078043
15 0.78073373
16 0.77750226
17 -0.93467319
18 0.09076329
19 0.06401809
20 -0.89251844
21 0.03676719
22 -0.00706945
23 0.68371916
24 -0.49900491
25 0.38170543
26 -0.39641669
27 1.36027557
28 -0.04913748
29 0.11267268
30 1.00445053
31 0.31684191

Total number of rows: 43376

Table truncated, full table size 727 Kbytes.




Supplementary file Size Download File type/resource
GSM1385015_US22502634_251485062126_S01_GE2-v5_95_Feb07_control_vs_CBD_3.txt.gz 15.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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