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Sample GSM1377566 Query DataSets for GSM1377566
Status Public on Jun 17, 2014
Title IgG NSC 6h
Sample type SRA
 
Source name rat NSC cells
Organism Rattus norvegicus
Characteristics cell type: Cortex derived Neural stem cells
pasage: Passage 2
chip antibody: IgG
treatment: FGF
time: 6 h
Treatment protocol ChIP seq was performed as described in methods. Full study is published as Castello-Branco et al (2014) Stem Cell reports
Growth protocol Neural Stem cells from disected cortex of E15,5 rat embryos were grown in the presense of FGF for 6h and 24h before fixation with formaldehyde
Extracted molecule genomic DNA
Extraction protocol Sonicated nuclear extracts were incubated with antibodies
Libraries were prepared according to Illumina's instructions accompanying the ChIP-Seq DNA Sample Prep Kit (IP-102-1001).
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing Reads were mapped to the rat genome build rn4 by Bowtie
peaks were generated using Cis Genome with input control as background.
Genome_build: rn4
Supplementary_files_format_and_content: BED
 
Submission date May 01, 2014
Last update date May 15, 2019
Contact name Ola Hermanson
E-mail(s) ola.hermanson@ki.se
Organization name Karolinska Institutet
Department Neuroscience
Lab Hermanson
Street address Retzius Väg 8
City Stockholm
ZIP/Postal code 17177
Country Sweden
 
Platform ID GPL14844
Series (1)
GSE57232 ChIP-seq experiment analyzing binding of HDAC2 and HDAC3 to genomic loci in rat Neural Stem Cells
Relations
BioSample SAMN02741880
SRA SRX530449

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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