|
| Status |
Public on Dec 31, 2014 |
| Title |
Relapse tumor E-3-off-Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Culture of relapse tumor E-3 off dox
|
| Organism |
Mus musculus |
| Characteristics |
strain background: FVB/B6 mix
genotype/variation: p48Cre tetO_LKrasG12D ROSA_rtTAL+ p53L+
cell line: E-3
cell type: primary relapsed pancreatic tumor cell line
treatment: Off Dox
|
| Treatment protocol |
Cells were grown in Tet-Free FBS (Clontech) in the presence or absence of doxycycline
|
| Growth protocol |
Cells were grown in Tet-Free FBS (Clontech) at 37C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNeasy column cleanup was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
E-3-2
Culture of relapse tumor E-3
|
| Data processing |
The data was RMA processed using Affy R package, The raw intensity values are background corrected, log2 transformed and then quantile normalized.
|
| |
|
| Submission date |
Apr 29, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Avnish Kapoor |
| E-mail(s) |
akapoor@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1901 East Rd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77025 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE53167 |
Yap1 activation enables bypass of oncogenic Kras addiction in pancreatic cancer (part 1) |
| GSE53169 |
Yap1 activation enables bypass of oncogenic Kras addiction in pancreatic cancer |
|
