|
| Status |
Public on Apr 29, 2014 |
| Title |
HaCaT obacunone 100 uM 1 set |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: keratinocyte
agent: obacunone 100 uM
|
| Treatment protocol |
Cells were seeded at a density of 1 x 106 cells in a 100 mm dish and incubated overnight. Next day, the cells were fed with fresh media containing 10% FBS and incubated with obacunone or 0.1% DMSO (vehicle control) for 4 h.
|
| Growth protocol |
Cells were maintained in DMEM supplemented with 10% FBS and antibiotics at 37°C in a humidified 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina® TotalPrep RNA Amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalized using quantile normalization with Avadis 3.3 software
|
| |
|
| Submission date |
Apr 28, 2014 |
| Last update date |
Apr 29, 2014 |
| Contact name |
Jong Min Kim |
| E-mail(s) |
indigo@eyegene.co.kr
|
| Organization name |
Sejong University
|
| Department |
Bioscience and Biotechnology
|
| Street address |
209, Neungdong-ro, Gwangjin-gu
|
| City |
Seoul |
| ZIP/Postal code |
143-747 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6104 |
| Series (2) |
| GSE57120 |
Gene expression profile analysis of human keratinocyte HaCaT cells treated with obacunone |
| GSE57121 |
Gene expression profiles of human keratinocytes and hepatocytes treated with obacunone |
|
