RNA was extracted from cell lines using the Qiagen RNeasy kit as per the manufacturer's instructions. RNA quantity was determined using a Nanodrop spectrophotometer and both 260/280 and 260/230 ratios were evaluated. RNA quality was assessed using an Agilent RNA 6000 Nano Chip and the RNA Integrity Number (RIN) value of each sample was determined.
The data were analysed with the robust multichip analysis algorithm. The data is returned in log2 normalised format. The RMA version used was from the bioconductor package affy, stable release version number 1.40.