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Sample GSM1374922 Query DataSets for GSM1374922
Status Public on Apr 28, 2014
Title Bx058-14 HG2
Sample type RNA
Organism Homo sapiens
Characteristics cell line: SW1783
tissue: Brain
disease: astrocytoma
Extracted molecule total RNA
Extraction protocol RNA was extracted from cell lines using the Qiagen RNeasy kit as per the manufacturer's instructions. RNA quantity was determined using a Nanodrop spectrophotometer and both 260/280 and 260/230 ratios were evaluated. RNA quality was assessed using an Agilent RNA 6000 Nano Chip and the RNA Integrity Number (RIN) value of each sample was determined.
Label biotin
Label protocol Affymetrix protocol
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Description Untreated
Data processing The data were analysed with the robust multichip analysis algorithm. The data is returned in log2 normalised format. The RMA version used was from the bioconductor package affy, stable release version number 1.40.
Submission date Apr 25, 2014
Last update date Apr 28, 2014
Contact name Mark Wappett
Organization name AstraZeneca
Department Oncology
Lab Bioinformatics
Street address Alderley Park
City Macclesfield
ZIP/Postal code SK10 4TG
Country United Kingdom
Platform ID GPL570
Series (1)
GSE57083 Expression data from AstraZeneca internal cell lines

Data table header descriptions
VALUE RMA log2 signal intensity

Data table
1007_s_at 9.69576025495086
1053_at 9.24250819549831
117_at 4.22267038149943
121_at 7.41192235038093
1255_g_at 2.72151019547987
1294_at 6.82051466004784
1316_at 5.93939667251841
1320_at 4.71186580584676
1405_i_at 7.40134237299806
1431_at 3.38077901482509
1438_at 5.16433233405755
1487_at 8.00554436741662
1494_f_at 5.31796414023233
1552256_a_at 8.56382599968014
1552257_a_at 8.18423170124327
1552258_at 5.39318274580932
1552261_at 4.34421619944278
1552263_at 6.68905046377446
1552264_a_at 6.98571459621262
1552266_at 3.63273893199591

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM1374922_Bx058-14_HG2.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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