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Sample GSM1363307 Query DataSets for GSM1363307
Status Public on Sep 01, 2014
Title P001
Sample type SRA
 
Source name placenta
Organism Homo sapiens
Characteristics person: child
trio: P001
Extracted molecule total RNA
Extraction protocol mirVana™ miRNA Isolation Kit (Ambion®, Life Technologies™) according to manufacturer’s instructions
Ovation RNA-Seq System V2 Kit (NuGen, Emeryville, CA, USA) + SOLiD 5500 System; DNA fragment library kit (Life Technologies, Carlsbad, CA, USA), both according to manufacturer's instructions.
45 ng total RNA was used to consruct WT RNA seq library (cDNA synthesis, cDNA amplification, SOLiDi 5500 adaptor ligation and library amplification), barcoded adaptors were applied for multiplex sequencing
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model AB 5500xl Genetic Analyzer
 
Data processing DESeq package in the R statistical language and environment (version 3.0.2) was used to calculate the reads per kilobase per million reads (RPKM)
Genome_build: hg19
Supplementary_files_format_and_content: Raw read counts and RPKM values for each sample
 
Submission date Apr 04, 2014
Last update date May 15, 2019
Contact name Tauno Metsalu
Organization name University of Tartu
Street address Liivi 2
City Tartu
ZIP/Postal code 50409
Country Estonia
 
Platform ID GPL16288
Series (2)
GSE56524 Using RNA sequencing for identifying gene imprinting and random monoallelic expression in human placenta (RNA-seq)
GSE56781 Using RNA sequencing for identifying gene imprinting and random monoallelic expression in human placenta
Relations
BioSample SAMN02720945
SRA SRX512270

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data is available on Series record

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