|
| Status |
Public on Oct 27, 2014 |
| Title |
Male Repeat of Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
LVP-IB12_WT_male_pupal heads
|
| Organism |
Aedes aegypti |
| Characteristics |
strain: Liverpool-IB12 (LVP-IB12)
genotype/variation: wild type
developmental stage: 24 hours after pupariaum formation
Sex: male
tissue: head
|
| Treatment protocol |
Following animal sexing, 20 age-matched male and female heads per replicate for each of four replicates were dissected from 24 hr pupae and immediately stored in RNAeasy (Qiagen) for subsequent RNA extraction.
|
| Growth protocol |
Mosquitoes were maintained in an insectary at 26° C, ~80% humidity, under a 12 hr light and 12 hr dark cycle with 1 hr crepuscular periods at the beginning and end of each light cycle. Mosquito larvae were fed a suspension of dried beef liver powder, while adults were provided cotton soaked with 10% sugar solution. An artificial membrane blood feeding system was utilized to deliver a sheep blood meal to females.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each of the four replicates, total RNA extraction was performed with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. Total RNA sample integrity was verified with a 2100 Bioanalyzer (Agilent Technologies, Inc.).
|
| Label |
Cy3-Random Nonamer
|
| Label protocol |
Labeling was performed by the Notre Dame Genomics and Bioinformatics Core Facility according to the NimbleGen Gene Expression Analysis Labeling Protocol v3.2.
|
| |
|
| Hybridization protocol |
Hybridization to the Roche NimbleGen 12-plex Aedes aegypti microarray, design: 090305_Aedes_aegypti_TEfam_expr.ndf, was performed in a GeneChip Hybridization Oven 640 according to the manufacturer’s instructions. Four unique replicates and two repeat replicates were assessed in the hybridization experiment.
|
| Scan protocol |
Microarrays were scanned using a NimbleGen MS 200 Microarray Scanner (Roche NimbleGen).
|
| Description |
SAMPLE 10
Gene expression data from male Aedes aegypti 24 hr pupal head replicate
|
| Data processing |
NimbleScan was used to calculate signal intensities.
Microarray data pre-processing and normalization were performed using the Bioconductor packages in R Version 3.0.1. Raw gene expression data were log-transformed and normalized using the quantile normalization method.
|
| |
|
| Submission date |
Apr 04, 2014 |
| Last update date |
Oct 27, 2014 |
| Contact name |
Molly Duman Scheel |
| Organization name |
Indiana University School of Medicine
|
| Street address |
1234 Notre Dame Ave.
|
| City |
South Bend |
| State/province |
IN |
| ZIP/Postal code |
46617 |
| Country |
USA |
| |
|
| Platform ID |
GPL18530 |
| Series (1) |
| GSE56521 |
Sex-specific gene expression profiles of Aedes aegypti 24 hr pupal heads |
|
