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Sample GSM135468 Query DataSets for GSM135468
Status Public on Feb 08, 2007
Title daf2_RepC
Sample type RNA
 
Source name daf2
Organism Caenorhabditis elegans
Characteristics Strain: daf-2(e1368)
Age: late L4/young adult
Tissue: whole animal
Growth protocol Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested.
Extracted molecule total RNA
Extraction protocol TRI Reagent (Molecular Research Center)
Label biotin
Label protocol Affymetrix
 
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested.
Data processing Resolver Gene Expression Data Analysis System, version 5.1 (Rosetta Inpharmatics)
 
Submission date Sep 08, 2006
Last update date Jun 23, 2008
Contact name Emily Troemel
E-mail(s) etroemel@ucsd.edu
Organization name University of California, San Diego
Department Cell and Developmental Biology
Lab Troemel
Street address 9500 Gilman Dr, #0349
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL200
Series (1)
GSE5801 Genes regulated by PMK-1 and DAF-16 in a daf-2(e1368) background.

Data table header descriptions
ID_REF
VALUE Intensity

Data table
ID_REF VALUE
171720_x_at 199.30319
171721_x_at 569.52576
171722_x_at 892.82062
171723_x_at 16457.22656
171724_x_at 1209.75793
171725_x_at 14411.12012
171726_x_at 46.20064
171727_x_at 9.75135
171728_x_at 64.46543
171729_x_at 462.01031
171730_x_at 21.64251
171731_x_at 826.72607
171732_x_at 523.65448
171733_x_at 17.68347
171734_x_at 4853.82373
171735_x_at 5757.04932
171736_x_at 109.27040
171737_x_at 162.50383
171738_x_at 596.50671
171739_x_at 134.97739

Total number of rows: 22625

Table truncated, full table size 441 Kbytes.




Supplementary file Size Download File type/resource
GSM135468.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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