|
| Status |
Public on Sep 01, 2014 |
| Title |
PyMT Tumor B |
| Sample type |
RNA |
| |
|
| Source name |
mouse mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N
genotype: PyMT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and the RNEasy mini kit (Qiagen) with on-column DNAse digestion was used following Trizol extraction. 100ng total RNA per sample was used for array analysis.
|
| Label |
biotin
|
| Label protocol |
Fragmented ssDNA was labeled with the Affymetrix WT Terminal Labeling and Hybridization kit according to the instructions, using Affymetrix proprietary biotinylated labeling reagent.
|
| |
|
| Hybridization protocol |
Samples were hybridized using the Gene Atlas Microarray System (Affymetrix) hybridization chamber; 20 hours at 48 C
|
| Scan protocol |
Samples were scanned and imaged using the Gene Atlas Microarray System Fludics and Imaging equipment (Affymetrix)
|
| Data processing |
CEL files were processed in Partek Genomic Suite using RMA Quantile normalization; intensity values were scaled by log2 transformation
|
| |
|
| Submission date |
Mar 13, 2014 |
| Last update date |
Sep 01, 2014 |
| Contact name |
Elizabeth A Wellberg |
| E-mail(s) |
elizabeth-wellberg@ouhsc.edu
|
| Organization name |
University of Oklahoma HSC
|
| Department |
Pathology
|
| Street address |
975 NE 10th St, BRC 309
|
| City |
Oklahoma City |
| State/province |
Oklahoma |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE55886 |
Effect of Spot14 Loss on Gene Expression Profile of MMTV-PyMT Mouse Tumors |
|
