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Sample GSM133945 Query DataSets for GSM133945
Status Public on Feb 16, 2007
Title Murray_2-1_T0-APH_Rep1_ATH1
Sample type RNA
Source name na
Organism Arabidopsis thaliana
Characteristics Developmental stage: early stationary phase
Tissue: cell suspension
Treatment protocol 20 ml of an early stationary phase MM2d cell suspension (7 days after previous subculture) was transferred into 100 ml fresh MSS-medium (MS-salt, 3% sucrose, 0.5mg/l NAA, 0.05mg/l kinetin) and incubated in the presence of 4.16 ug/ml aphidicolin (not exp.variable) for 24 hours under continuous darkness by rotating at 130 rpm at a temperature of 27C. Cells were gently washed with 1 l MSS-medium through a nylon net (47um mesh size), followed by centrifugation (387 x g for 1 min, no brake applied) to remove the drug. Cells were resuspended in a total volume of 120 ml fresh MSS-medium, incubated as above and samples were taken 2-3 hourly for analysis. The T0 sample was taken directly after washing to release the block (exp. variable; time course).
Extracted molecule total RNA
Label biotin
Description DNA replication inhibitor: aphidicolin
growth media type: liquid culture media
light regime: continuous darkness
nutrients: 100 ml MS salts, 3% sucrose, 0.5 mg/l NAA, 0.05 mg/l kinetin
average daily temperature: 27oC
Data processing The data were analysed using Affymetrix Microarray Suite 5.0 (MAS5)
Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platform ID GPL198
Series (1)
GSE5747 Genome-wide cell cycle studies
Reanalyzed by GSE119083

Data table header descriptions
VALUE This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL A qualitative measurement indicating if the probe set is detected
DETECTION P-VALUE A p-value indicating the significance of the Detection call

Data table
AFFX-r2-P1-cre-5_at 1.71530468750000E+003 P 2.44141003349796E-004
AFFX-r2-P1-cre-3_at 2.12750903320313E+003 P 2.44141003349796E-004
AFFX-r2-Ec-bioD-5_at 2.60019165039063E+002 P 7.32421991415322E-004
AFFX-r2-Ec-bioD-3_at 3.41991119384766E+002 P 2.44141003349796E-004
AFFX-r2-Ec-bioC-5_at 6.74287033081055E+001 P 7.32421991415322E-004
AFFX-r2-Ec-bioC-3_at 1.13255516052246E+002 P 2.44141003349796E-004
AFFX-r2-Ec-bioB-M_at 4.07081031799316E+001 P 1.95312988944352E-003
AFFX-r2-Ec-bioB-5_at 4.01830787658691E+001 P 7.32421991415322E-004
AFFX-r2-Ec-bioB-3_at 4.43937492370605E+001 P 1.41602000221610E-002
AFFX-r2-Bs-thr-M_s_at 3.48780131340027E+000 A 5.67627012729645E-001
AFFX-r2-Bs-thr-5_s_at 1.46274042129517E+001 M 5.61522990465164E-002
AFFX-r2-Bs-thr-3_s_at 8.08657169342041E-001 A 8.50341975688934E-001
AFFX-r2-Bs-phe-M_at 1.17004323005676E+000 A 5.33936023712158E-001
AFFX-r2-Bs-phe-5_at 6.42413187026978E+000 A 1.71387001872063E-001
AFFX-r2-Bs-phe-3_at 7.76643133163452E+000 A 3.03710997104645E-001
AFFX-r2-Bs-lys-M_at 3.95803242921829E-001 A 7.25830018520355E-001
AFFX-r2-Bs-lys-5_at 2.53661656379700E+000 A 4.66064006090164E-001
AFFX-r2-Bs-lys-3_at 1.25831699371338E+001 M 5.61522990465164E-002
AFFX-r2-Bs-dap-M_at 9.95945453643799E-001 A 6.01073980331421E-001
AFFX-r2-Bs-dap-5_at 3.32851767539978E-001 A 8.28612983226776E-001

Total number of rows: 22810

Table truncated, full table size 1250 Kbytes.

Supplementary file Size Download File type/resource
GSM133945.CEL.gz 2.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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