|
| Status |
Public on Jul 23, 2015 |
| Title |
Runx1f/f primary myoblasts, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
141
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Runx1 f/f
cell type: Myobalst
genetic background: C57bl/6
|
| Treatment protocol |
comparison of WT to KO PM
|
| Growth protocol |
We isolatedf SC from gastrocnemius muscles of 2-3 weeks old C57bl/6 WT or Runx1f/f mice. Cells were grown at 37°C, 5% CO2 on GHR MatrigelTM (BD Biosciences, USA) in proliferation medium (BIOAMF-2, Biological Industries, Israel), which was replaced daily
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by PerfectPure RNA tissue kit (# 2302410, 5 PRIME, Germany) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
200ng TotalRNA were used for labeling. cDNA samples were generated and labeled using Affymetrix WT-cDNA synthesis and labeling kit
|
| |
|
| Hybridization protocol |
2ug ss cDNA in 80 ul total volume were hybridized to a Mouse Gene 1.0 ST array using Affymetrix hybridization kit materials and hybridized for 17 hours at 45° at 60rpm. Post-hyb washes were done with Affymetrix wash solutions on the FS450 station using FS450_0007 protocol.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The analysis was carried out using Partek Genome Suite software (Partek Inc., St. Louis, Missouri 63141, http://www.partek.com), using RMA.
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
Jul 23, 2015 |
| Contact name |
kfir baruch Umansky |
| E-mail(s) |
kfirum@weizmann.ac.il
|
| Phone |
97289342318
|
| Organization name |
Weizmann Institute
|
| Street address |
POB 25
|
| City |
Rehovot |
| State/province |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE55556 |
Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation [Expression Study] |
| GSE56131 |
Transcription factor Runx1 cooperates with MyoD and c-Jun to regulate the balance of myoblast proliferation/differentiation |
|
