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Sample GSM133718 Query DataSets for GSM133718
Status Public on Jan 08, 2007
Title Urwin_A-2-Urwin-Inf_SLD
Sample type RNA
Source name Col-0
Organism Arabidopsis thaliana
Characteristics Stock Code: N1093
Developmental stage: 6.1
Tissue: lateral roots
Treatment protocol At 21 days germination (Growth Stage 3.4/3.5) Arabidopsis plants were challenged with rigorously sterilised,infective nematodes of H. schachtii as before (Urwin et al., (1997) Plant Journal 12: 455-461). 35 sterile J2s were pipetted onto small ~0.5 mm2 squares of sterile GF/A filter paper. The GF/A paper was left in direct contact with the zone of elongation on 3 lateral roots per plant for 48 hours. Sections of root containing syncytia have been excised from the thin and transparent roots of Arabidopsis and collected into RNAlater solution (Ambion) at 21 days post infection (Growth Stage 6.1). The female nematode has been removed.
Extracted molecule total RNA
Label biotin
Description location: Sanyo MLR3500 growth cabinet
Atmosphere: Normal
Humidity: Ambient
Temperature: 20.5
Lighting: 16:8 light:dark
Medium: ½MS10 media 0.2 % phytagel
Treatment: n/a
Nutrients: ½MS10 media
Water: n/a
Stratification: on moist filter paper @ 4 oC for 3 days
Data processing The data were analysed using Affymetrix Microarray Suite 5.0 (MAS5)
Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
Platform ID GPL198
Series (1)
GSE5724 Plant gene expression associated with susceptibility to nematodes
Reanalyzed by GSE119083

Data table header descriptions
VALUE This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL A qualitative measurement indicating if the probe set is detected
DETECTION P-VALUE A p-value indicating the significance of the Detection call

Data table
AFFX-r2-P1-cre-5_at 7.73290000000000E+003 P 2.44000000000000E-004
AFFX-r2-P1-cre-3_at 7.96740000000000E+003 P 2.44000000000000E-004
AFFX-r2-Ec-bioD-5_at 1.84010000000000E+003 P 2.44000000000000E-004
AFFX-r2-Ec-bioD-3_at 1.90060000000000E+003 P 2.44000000000000E-004
AFFX-r2-Ec-bioC-5_at 4.18400000000000E+002 P 2.44000000000000E-004
AFFX-r2-Ec-bioC-3_at 5.36600000000000E+002 P 2.44000000000000E-004
AFFX-r2-Ec-bioB-M_at 2.03400000000000E+002 P 2.44000000000000E-004
AFFX-r2-Ec-bioB-5_at 1.48800000000000E+002 P 7.32000000000000E-004
AFFX-r2-Ec-bioB-3_at 1.63200000000000E+002 P 2.44000000000000E-004
AFFX-r2-Bs-thr-M_s_at 2.59000000000000E+001 A 3.66211000000000E-001
AFFX-r2-Bs-thr-5_s_at 2.42000000000000E+001 A 1.29639000000000E-001
AFFX-r2-Bs-thr-3_s_at 5.50000000000000E+000 A 6.33789000000000E-001
AFFX-r2-Bs-phe-M_at 5.80000000000000E+000 A 3.98926000000000E-001
AFFX-r2-Bs-phe-5_at 4.30000000000000E+000 A 5.33936000000000E-001
AFFX-r2-Bs-phe-3_at 3.90000000000000E+000 A 6.65527000000000E-001
AFFX-r2-Bs-lys-M_at 8.50000000000000E+000 A 5.00000000000000E-001
AFFX-r2-Bs-lys-5_at 1.10000000000000E+000 A 8.50342000000000E-001
AFFX-r2-Bs-lys-3_at 5.40000000000000E+000 A 2.74170000000000E-001
AFFX-r2-Bs-dap-M_at 7.00000000000000E-001 A 6.96289000000000E-001
AFFX-r2-Bs-dap-5_at 7.10000000000000E+000 A 6.96289000000000E-001

Total number of rows: 22810

Table truncated, full table size 1250 Kbytes.

Supplementary file Size Download File type/resource
GSM133718.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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