|
| Status |
Public on Jan 08, 2007 |
| Title |
Urwin_A-2-Urwin-Inf_SLD |
| Sample type |
RNA |
| |
|
| Source name |
Col-0
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Stock Code: N1093
Developmental stage: 6.1
Tissue: lateral roots
|
| Treatment protocol |
At 21 days germination (Growth Stage 3.4/3.5) Arabidopsis plants were challenged with rigorously sterilised,infective nematodes of H. schachtii as before (Urwin et al., (1997) Plant Journal 12: 455-461). 35 sterile J2s were pipetted onto small ~0.5 mm2 squares of sterile GF/A filter paper. The GF/A paper was left in direct contact with the zone of elongation on 3 lateral roots per plant for 48 hours. Sections of root containing syncytia have been excised from the thin and transparent roots of Arabidopsis and collected into RNAlater solution (Ambion) at 21 days post infection (Growth Stage 6.1). The female nematode has been removed.
|
| Extracted molecule |
total RNA |
| Label |
biotin
|
| |
|
| Description |
location: Sanyo MLR3500 growth cabinet
Atmosphere: Normal
Humidity: Ambient
Temperature: 20.5
Lighting: 16:8 light:dark
Medium: ½MS10 media 0.2 % phytagel
Treatment: n/a
Nutrients: ½MS10 media
Water: n/a
Stratification: on moist filter paper @ 4 oC for 3 days
|
| Data processing |
The data were analysed using Affymetrix Microarray Suite 5.0 (MAS5)
|
| |
|
| Submission date |
Sep 01, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE5724 |
Plant gene expression associated with susceptibility to nematodes |
|
| Relations |
| Reanalyzed by |
GSE119083 |
