|
| Status |
Public on Apr 03, 2014 |
| Title |
ChIP-Seq Analysis of PC in S2 cells, first replicate |
| Sample type |
SRA |
| |
|
| Source name |
PC 1
|
| Organism |
Drosophila melanogaster |
| Characteristics |
chip antibody: Polycomb
cell line: S2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Purified ChIP-DNA was prepared for sequencing on a HiSeq 2000 sequencer using Illumina standard protocol.
TruSeq ChIP Sample Preparation Kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalling, CASAVA 1.8.2, Illumina
Alignment on dm3, CASAVA 1.8.2, Illumina
Quality measures with FastQC
Peak calling using MACS 1.4.2
Assignment to genes using Perl scripts
Genome_build: dm3
Supplementary_files_format_and_content: bed peak files
|
| |
|
| Submission date |
Feb 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Giacomo Cavalli |
| E-mail(s) |
giacomo.cavalli@igh.cnrs.fr
|
| Organization name |
CNRS
|
| Lab |
INSTITUTE of HUMAN GENETICS
|
| Street address |
IGH - CNRS, 141, rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 Cedex5 |
| Country |
France |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE55303 |
Identification of new regulators of the three dimensional Polycomb organization by a microscopy-based genome-wide RNAi screen |
|
| Relations |
| BioSample |
SAMN02650909 |
| SRA |
SRX475424 |
