|
| Status |
Public on Sep 08, 2014 |
| Title |
CRE3.K79me1 |
| Sample type |
SRA |
| |
|
| Source name |
Leukemic bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
tissue: bone marrow
diagnosis: leukemia
chip antibody: Ab3261 Abcam
|
| Treatment protocol |
Standard formaldehyde fixation (1%)
|
| Growth protocol |
leukemic BM cells cultured in DMEM 15%FBS + pen/strep and 10ng/ml IL6, 20ng/ml SCF, 6ng/ml IL3
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Standard Illumina TruSeq DNA sample preparation protocol for the library prep
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequence reads were aligned to genome assembly mm9 using BWA with default parameters.
Wiggle (wig) files were generated using a using a custom pipeline.
Genome_build: mm9
Supplementary_files_format_and_content: The wiggle file contains average read density over the whole genome. (http://genome.ucsc.edu/goldenPath/help/wiggle.html)
|
| |
|
| Submission date |
Feb 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Scott Armstrong |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE54500 |
Role of H3K79 methylation states in HOX gene expression and leukemogenesis |
| GSE55038 |
Genome wide mapping of histone 3 lysine 79 mono, di and trimethylation and histone 3 lysine 27 trimethylation in LSKs, GMPs and retroviral MLL-AF9 driven murine leukemias |
|
| Relations |
| BioSample |
SAMN02642529 |
| SRA |
SRX471246 |
