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Sample GSM1328206 Query DataSets for GSM1328206
Status Public on Jun 05, 2015
Title Patient 3 - skin-week 3/4 visit
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics treatment: fresolimumab two doses 1 mg/kg at baseline and 3 wks
Extracted molecule total RNA
Extraction protocol Skin biopseie placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RNAzol and prifying over Rneasy columns
Label biotin
Label protocol Biotin labeling was performed using the 3' IVT Express Kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Feb 14, 2014
Last update date Jun 05, 2015
Contact name Robert Lafyatis
E-mail(s) rlafyatis@gmail.com
Phone 6176385388
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE55036 Expression data from skin biopsies in pateints with systemic sclerosis treated with anti-TGF-beta, fresolimumab

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 974.8765
1053_at 466.5414
117_at 174.1391
121_at 193.1172
1255_g_at 22.7224
1294_at 83.18698
1316_at 60.60561
1320_at 189.2558
1405_i_at 136.2876
1431_at 242.0549
1438_at 102.8369
1487_at 232.537
1494_f_at 29.77242
1598_g_at 1451.148
160020_at 485.8331
1729_at 366.3579
1773_at 85.39603
177_at 209.978
179_at 126.0346
1861_at 193.8779

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.




Supplementary file Size Download File type/resource
GSM1328206_SD-11-15.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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