|
| Status |
Public on Mar 10, 2014 |
| Title |
Wildtype E8 |
| Sample type |
SRA |
| |
|
| Source name |
embryos
|
| Organism |
Mus musculus |
| Characteristics |
mouse strain of origin: (C3H/HeH x 101/H)F1
developmental stage: E7.5 ~ 8.0
genotype: Wildtype
tissue: embryos
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol LS Reagent (Invitrogen)
Libraries were prepared with ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Directional mRNA-seq
|
| Data processing |
Reads Mapping: Bowtie 2
Differential Gene expression analysis: Cuffdiff
Significance Thresholding: R
Genome_build: mm9
Supplementary_files_format_and_content: Table containing differential gene expression information
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhe Liu |
| E-mail(s) |
liuz11@janelia.hhmi.org
|
| Organization name |
Howard Hughes Medical Institute
|
| Department |
Janelia Farm Research Campus
|
| Lab |
Liu
|
| Street address |
19700 Helix Dr.
|
| City |
Ashburn |
| State/province |
Virginia |
| ZIP/Postal code |
20147 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE54982 |
Probing In vivo Brachyury Function with Tc mouse model |
| GSE54985 |
Charting Brachyury-mediated Developmental Pathways During Early Mouse Embryogenesis |
|
| Relations |
| BioSample |
SAMN02641392 |
| SRA |
SRX470251 |
