|
| Status |
Public on Sep 30, 2014 |
| Title |
Liv_CT38 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
tissue: liver
|
| Growth protocol |
C57/BL6 mice were entrained to a 12h light : 12h dark schedule for one week. Mice were then transferred to complete darkness prior to collection. Collection began at CT18. Mice were housed in light-tight boxes for the duration of the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Organ samples were homogenized in Trizol reagent (Invitrogen) using a Tissuelyser (Qiagen). RNA was extracted using RNeasy columns (Qiagen) as per manufacturer’s protocol, then pooled from three mice for each organ and time point.
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
not provided
|
| Data processing |
Intensity values normalized using Affymetrix Expression Console software (GC-RMA).
|
| |
|
| Submission date |
Feb 04, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Nicholas Lahens |
| Organization name |
University of Pennsylvania
|
| Department |
ITMAT
|
| Street address |
Smilow Center for Translational Research 10-110 3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE54650 |
A circadian gene expression atlas in mammals assayed by microarray |
| GSE54652 |
A circadian gene expression atlas in mammals: Implications for biology and medicine |
|
