|
Status |
Public on Sep 30, 2014 |
Title |
Hyp_CT52 |
Sample type |
RNA |
|
|
Source name |
hypothalamus
|
Organism |
Mus musculus |
Characteristics |
strain: C57/BL6 tissue: hypothalamus
|
Growth protocol |
C57/BL6 mice were entrained to a 12h light : 12h dark schedule for one week. Mice were then transferred to complete darkness prior to collection. Collection began at CT18. Mice were housed in light-tight boxes for the duration of the experiment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Organ samples were homogenized in Trizol reagent (Invitrogen) using a Tissuelyser (Qiagen). RNA was extracted using RNeasy columns (Qiagen) as per manufacturer’s protocol, then pooled from three mice for each organ and time point.
|
Label |
biotin
|
Label protocol |
not provided
|
|
|
Hybridization protocol |
not provided
|
Scan protocol |
not provided
|
Data processing |
Intensity values normalized using Affymetrix Expression Console software (GC-RMA).
|
|
|
Submission date |
Feb 04, 2014 |
Last update date |
Sep 30, 2014 |
Contact name |
Nicholas Lahens |
Organization name |
University of Pennsylvania
|
Department |
ITMAT
|
Street address |
Smilow Center for Translational Research 10-110 3400 Civic Center Blvd, Bldg 421
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE54650 |
A circadian gene expression atlas in mammals assayed by microarray |
GSE54652 |
A circadian gene expression atlas in mammals: Implications for biology and medicine |
|