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Sample GSM1321151 Query DataSets for GSM1321151
Status Public on Sep 30, 2014
Title Hyp_CT52
Sample type RNA
 
Source name hypothalamus
Organism Mus musculus
Characteristics strain: C57/BL6
tissue: hypothalamus
Growth protocol C57/BL6 mice were entrained to a 12h light : 12h dark schedule for one week. Mice were then transferred to complete darkness prior to collection. Collection began at CT18. Mice were housed in light-tight boxes for the duration of the experiment.
Extracted molecule total RNA
Extraction protocol Organ samples were homogenized in Trizol reagent (Invitrogen) using a Tissuelyser (Qiagen). RNA was extracted using RNeasy columns (Qiagen) as per manufacturer’s protocol, then pooled from three mice for each organ and time point.
Label biotin
Label protocol not provided
 
Hybridization protocol not provided
Scan protocol not provided
Data processing Intensity values normalized using Affymetrix Expression Console software (GC-RMA).
 
Submission date Feb 04, 2014
Last update date Sep 30, 2014
Contact name Nicholas Lahens
Organization name University of Pennsylvania
Department ITMAT
Street address Smilow Center for Translational Research 10-110 3400 Civic Center Blvd, Bldg 421
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL6246
Series (2)
GSE54650 A circadian gene expression atlas in mammals assayed by microarray
GSE54652 A circadian gene expression atlas in mammals: Implications for biology and medicine

Data table header descriptions
ID_REF
VALUE Normalized GC-RMA intensity values

Data table
ID_REF VALUE
10338001 2893.2
10338002 64
10338003 985.5
10338004 406.1
10338005 15.4
10338006 16.8
10338007 19.4
10338008 23.4
10338009 261.3
10338010 15.8
10338011 43
10338012 16.3
10338013 14.2
10338014 14.8
10338015 14.4
10338016 152.1
10338017 6205.5
10338018 85.1
10338019 36.6
10338020 254.7

Total number of rows: 35556

Table truncated, full table size 499 Kbytes.




Supplementary file Size Download File type/resource
GSM1321151_Hypo52_MoGene1.0ST.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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