|
| Status |
Public on May 21, 2015 |
| Title |
undifferentiated embryonic stem cells, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated murine embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mESC
cell line: CGR8
age: day 0
differentiation medium: 10% GMEM
|
| Treatment protocol |
samples at different stages of differentiation were collected. Embryoid bodies were collected and washed 1 time in PBS and placed into TRIzol(Gibco)
|
| Growth protocol |
murine embryonic stem cells CGR8 were differentiated in hanging drop method.First cell were dissociated and grown in hanging drop with 500 cells per 25 μL .After Day 2 formed embryoid bodies were transfered to shaker and cultured addtional 3 days upon horizontal agitation. At chosen time points samples were collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Gene-Chip Scanner-3000-7G
|
| Description |
Gene expression data from undifferentiated mESC
|
| Data processing |
Robust Multi-array Analysis algorithm (RMA) did background correction, summarization and normalization.Quantile normalization method was employed to normalize the raw dataset at probe feature level
|
| |
|
| Submission date |
Jan 21, 2014 |
| Last update date |
May 21, 2015 |
| Contact name |
Dina Ivaniuk |
| E-mail(s) |
dina.ivanyuk@mail.ru
|
| Organization name |
Istitute for Neurophysiology University of Cologne
|
| Street address |
Robert Koch Str.39
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE54242 |
Expression data from differentiating murine embryonic stem cells |
|
