|
| Status |
Public on Mar 31, 2014 |
| Title |
BMDM, untreated, 1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow derived macrophages, untreated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: bone marrow
cell type: macrophages
treatment: Untreated
|
| Treatment protocol |
Bone marrow derived macrophages from C56BL/6J mice were either untreated or treated with 10 ng/ml of IFNg , LPS or 10 ng/ml of IFNg+10 ng/ml of LPS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The samples were harvested after 24h and RNA was isolated using Qiagen RNeasy mini kit.
|
| Label |
Biotin
|
| Label protocol |
Preparation of cRNA was done using Affymetrix 3'IVT Express kit and protocol provided by Affymetrix. Briefly, 0.5 ug of total RNA was converted into double-stranded cDNA and used to generate biotin labeled cRNA which was then purified.
|
| |
|
| Hybridization protocol |
12.5 micrograms of cRNA was fragmented and hybridized to Mouse Genome 430 2.0 arrays at 45°C for 19 hours in a rotisserie oven set at 60 rpm.
|
| Scan protocol |
Arrays were washed and stained using Affymrtrix HWS kit using their Fluidics station and then scanned on GeneChip scanner 3000.
|
| Description |
Gene expression data from untreated bone marrow derived macrophages
|
| Data processing |
Command Console/Expression Console/ExpressionStat5.0
|
| |
|
| Submission date |
Jan 10, 2014 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Jason A Hackney |
| E-mail(s) |
hackney.jason@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Department of Bioinformatics and Computational Biology
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE53986 |
NRROS negatively regulates ROS in phagocytes during host defense and autoimmunity |
|
