|
| Status |
Public on Mar 21, 2014 |
| Title |
Btk-2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow B-cell fraction, B220+CD19+ pre-B
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
cell type: pre-B cell
genotype/variation: Btk KO (VH81x transgenic Rag1-/-)
|
| Treatment protocol |
RNA was extracted after one PBS wash
|
| Growth protocol |
developing B cells were isolated directly from the bone marrow by FACS purification and processed for analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using the Affymetrix GeneChip® WT (Whole Transcript) Terminal Labeling Kit, according to manufacturers instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the mouse gene 1.0st array in a GeneChip Hyb oven 640 according to manufacturer instructions. Washing was done in a GeneChip WS-system according to manufacturer instructions.
|
| Scan protocol |
Scanning was done on a geneChip scanner 7G incl. autocaroussel using affymetrix launcher software for downstream image processing
|
| Description |
pre-B cells, from VH81x transgenic Rag1-/- mice
|
| Data processing |
Normalized gene expression values were calculated using robust multi-array average (RMA)
|
| |
|
| Submission date |
Jan 08, 2014 |
| Last update date |
Mar 21, 2014 |
| Contact name |
Ralph Stadhouders |
| E-mail(s) |
stadhoudersralph@gmail.com
|
| Organization name |
Erasmus Medical Center
|
| Department |
Pulmonary Medicine
|
| Street address |
Dr. Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE53896 |
Pre-BCR Signaling induce IgK Locus Accessibility by functional redistribution of Enhancer-mediated chromatin Interactions |
|
